ArticleBiophysical journal2000
Effects of imperatoxin A on local sarcoplasmic reticulum Ca(2+) release in frog skeletal muscle.
Article in Biophysical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 58 citations in OpenAlex.
- Novel Ca2+ wave mechanisms in cardiac myocytes revealed by multiscale Ca2+ release model.The Journal of general physiology · 2025Article
- Excitation-contraction coupling in mammalian skeletal muscle: Blending old and last-decade research.Frontiers in physiology · 2022Review
- Improper Remodeling of Organelles Deputed to CaInternational journal of molecular sciences · 2021Review
- Structure-function relationships of peptides forming the calcin family of ryanodine receptor ligands.The Journal of general physiology · 2016Article
- Long-Lasting Sparks: Multi-Metastability and Release Competition in the Calcium Release Unit Network.PLoS computational biology · 2016Article
- The excitation-contraction coupling mechanism in skeletal muscle.Biophysical reviews · 2014Review
- Scorpion venom components that affect ion-channels function.Toxicon : official journal of the International Society on Toxinology · 2013Review
- Both basic and acidic amino acid residues of IpTx(a) are involved in triggering substate of RyR1.Journal of biomedicine & biotechnology · 2011Article
- Ryanodol action on calcium sparks in ventricular myocytes.Pflugers Archiv : European journal of physiology · 2010Article
- Role of ryanodine receptor subtypes in initiation and formation of calcium sparks in arterial smooth muscle: comparison with striated muscle.Journal of biomedicine & biotechnology · 2009Review
- Charged surface area of maurocalcine determines its interaction with the skeletal ryanodine receptor.Biophysical journal · 2008Article
- Sparks and embers of skeletal muscle: the exciting events of contractile activation.Pflugers Archiv : European journal of physiology · 2007Review
- Maurocalcine interacts with the cardiac ryanodine receptor without inducing channel modification.The Biochemical journal · 2007Article
- Transient loss of voltage control of Ca2+ release in the presence of maurocalcine in skeletal muscle.Biophysical journal · 2006Article
- Automated detection of elementary calcium release events using the á trous wavelet transform.Biophysical journal · 2006Article
- Effects of tetracaine on voltage-activated calcium sparks in frog intact skeletal muscle fibers.The Journal of general physiology · 2006Article
- Differential effects of maurocalcine on Ca2+ release events and depolarization-induced Ca2+ release in rat skeletal muscle.The Journal of physiology · 2005Article
- Effects of ryanoids on spontaneous and depolarization-evoked calcium release events in frog muscle.Biophysical journal · 2004Article
- Altered elementary calcium release events and enhanced calcium release by thymol in rat skeletal muscle.Biophysical journal · 2004Article
- Localised calcium release events in cells from the muscle of guinea-pig gastric fundus.The Journal of physiology · 2004Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
We have investigated the effects of imperatoxin A (IpTx(a)) on local calcium release events in permeabilized frog skeletal muscle fibers, using laser scanning confocal microscopy in linescan mode. IpTx(a) induced the appearance of Ca(2+) release events from the sarcoplasmic reticulum that are approximately 2 s and have a smaller amplitude (31 +/- 2%) than the "Ca(2+) sparks" normally seen in the absence of toxin. The frequency of occurrence of long-duration imperatoxin-induced Ca(2+) release events increased in proportion to IpTx(a) concentrations ranging from 10 nM to 50 nM. The mean duration of imperatoxin-induced events in muscle fibers was independent of toxin concentration and agreed closely with the channel open time in experiments on isolated frog ryanodine receptors (RyRs) reconstituted in planar lipid bilayer, where IpTx(a) induced opening of single Ca(2+) release channels to prolonged subconductance states. These results suggest involvement of a single molecule of IpTx(a) in the activation of a single Ca(2+) release channel to produce a long-duration event. Assuming the ratio of full conductance to subconductance to be the same in the fibers as in bilayer, the amplitude of a spark relative to the long event indicates involvement of at most four RyR Ca(2+) release channels in the production of short-duration Ca(2+) sparks.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.