ArticleBiophysical journal2000
A micromechanic study of cell polarity and plasma membrane cell body coupling in Dictyostelium.
Article in Biophysical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed, 116 citations in OpenAlex.
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- Actin binding domain of filamin distinguishes posterior from anterior actin filaments in migratingBiophysics and physicobiology · 2016Article
- A resilient formin-derived cortical actin meshwork in the rear drives actomyosin-based motility in 2D confinement.Nature communications · 2015Article
- Blood and immune cell engineering: Cytoskeletal contractility and nuclear rheology impact cell lineage and localization: Biophysical regulation of hematopoietic differentiation and trafficking.BioEssays : news and reviews in molecular, cellular and developmental biology · 2015Review
- Model for probing membrane-cortex adhesion by micropipette aspiration and fluctuation spectroscopy.Biophysical journal · 2015Article
- Physical view on migration modes.Cell adhesion & migration · 2015Review
- Autonomous buckling of micrometer-sized lipid-protein membrane patches constructed by Dictyostelium discoideum.Journal of biological engineering · 2015Article
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- The Application of Micropipette Aspiration in Molecular Mechanics of Single Cells.Journal of nanotechnology in engineering and medicine · 2014Review
- Contractile forces sustain and polarize hematopoiesis from stem and progenitor cells.Cell stem cell · 2014Article
- Mechanical force sensing in tissues.Progress in molecular biology and translational science · 2014Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We used micropipettes to aspirate leading and trailing edges of wild-type and mutant cells of Dictyostelium discoideum. Mutants were lacking either myosin II or talin, or both proteins simultaneously. Talin is a plasma membrane-associated protein important for the coupling between membrane and actin cortex, whereas myosin II is a cytoplasmic motor protein essential for the locomotion of Dictyostelium cells. Aspiration into the pipette occurred above a threshold pressure only. For all cells containing talin this threshold was significantly lower at the leading edge of an advancing cell as compared to its rear end, whereas we found no such difference in cells lacking talin. Wild-type and talin-deficient cells were able to retract from the pipette against an applied suction pressure. In these cells, retraction was preceded by an accumulation of myosin II in the tip of the aspirated cell lobe. Mutants lacking myosin II could not retract, even if the suction pressures were removed after aspiration. We interpreted the initial instability and the subsequent plastic deformation of the cell surface during aspiration in terms of a fracture between the cell plasma membrane and the cell body, which may involve destruction of part of the cortex. Models are presented that characterize the coupling strength between membrane and cell body by a surface energy sigma. We find sigma approximately 0.6(1.6) mJ/m(2) at the leading (trailing) edge of wild-type cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.