ArticleThe Journal of general physiology2000
Extracellular ATP inhibits the small-conductance K channel on the apical membrane of the cortical collecting duct from mouse kidney.
Article in The Journal of general physiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 68 citations in OpenAlex.
- Extracellular Nucleotides and P2 Receptors in Renal Function.Physiological reviews · 2020Review
- Epithelial transport inThe Journal of general physiology · 2017Review
- Large-conductance CaThe Journal of physiology · 2017Article
- Romk1 Knockout Mice Do Not Produce Bartter Phenotype but Exhibit Impaired K Excretion.The Journal of biological chemistry · 2016Article
- Article
- Collecting duct principal cell transport processes and their regulation.Clinical journal of the American Society of Nephrology : CJASN · 2015Review
- P2Y2 receptor activation inhibits the expression of the sodium-chloride cotransporter NCC in distal convoluted tubule cells.Pflugers Archiv : European journal of physiology · 2014Article
- Angiotensin II type 2 receptor regulates ROMK-like K⁺ channel activity in the renal cortical collecting duct during high dietary K⁺ adaptation.American journal of physiology. Renal physiology · 2014Article
- Emerging role of the calcium-activated, small conductance, SK3 K+ channel in distal tubule function: regulation by TRPV4.PloS one · 2014Article
- P2Y2 receptor knock-out mice display normal NaCl absorption in medullary thick ascending limb.Frontiers in physiology · 2013Article
- A new look at electrolyte transport in the distal tubule.Annual review of physiology · 2012Review
- P2Y receptors and kidney function.Wiley interdisciplinary reviews. Membrane transport and signaling · 2012Article
- Regulation of renal NaCl and water transport by the ATP/UTP/P2Y2 receptor system.American journal of physiology. Renal physiology · 2011Review
- Coupled ATP and potassium efflux from intercalated cells.American journal of physiology. Renal physiology · 2011Article
- Pharmacological properties and physiological function of a P2X-like current in single proximal tubule cells isolated from frog kidney.The Journal of membrane biology · 2010Article
- Dietary Na+ inhibits the open probability of the epithelial sodium channel in the kidney by enhancing apical P2Y2-receptor tone.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2010Article
- Effects of extracellular nucleotides on renal tubular solute transport.Purinergic signalling · 2009Article
- Extracellular ATP inhibits transport in medullary thick ascending limbs: role of P2X receptors.American journal of physiology. Renal physiology · 2009Article
- Connexin 30 deficiency impairs renal tubular ATP release and pressure natriuresis.Journal of the American Society of Nephrology : JASN · 2009Article
- Homeostatic synapse-driven membrane plasticity in nucleus accumbens neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
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4 authors at 2 institutions in 1 country.
Funding
Abstract
We have used the patch-clamp technique to study the effects of changing extracellular ATP concentration on the activity of the small-conductance potassium channel (SK) on the apical membrane of the mouse cortical collecting duct. In cell-attached patches, the channel conductance and kinetics were similar to its rat homologue. Addition of ATP to the bathing solution of split-open single cortical collecting ducts inhibited SK activity. The inhibition of the channel by ATP was reversible, concentration dependent (K(i) = 64 microM), and could be completely prevented by pretreatment with suramin, a specific purinergic receptor (P(2)) blocker. Ranking of the inhibitory potency of several nucleotides showed strong inhibition by ATP, UTP, and ATP-gamma-S, whereas alpha, beta-Me ATP, and 2-Mes ATP failed to affect channel activity. This nucleotide sensitivity is consistent with P(2)Y(2) purinergic receptors mediating the inhibition of SK by ATP. Single channel analysis further demonstrated that the inhibitory effects of ATP could be elicited through activation of apical receptors. Moreover, the observation that fluoride mimicked the inhibitory action of ATP suggests the activation of G proteins during purinergic receptor stimulation. Channel inhibition by ATP was not affected by blocking phospholipase C and protein kinase C. However, whereas cAMP prevented channel blocking by ATP, blocking protein kinase A failed to abolish the inhibitory effects of ATP. The reduction of K channel activity by ATP could be prevented by okadaic acid, an inhibitor of protein phosphatases, and KT5823, an agent that blocks protein kinase G. Moreover, the effect of ATP was mimicked by cGMP and blocked by L-NAME (N(G)-nitro-l-arginine methyl ester). We conclude that the inhibitory effect of ATP on the apical K channel is mediated by stimulation of P(2)Y(2) receptors and results from increasing dephosphorylation by enhancing PKG-sensitive phosphatase activity.
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