ArticleApplied and environmental microbiology2000
Characterization of an isolate that uses vinyl chloride as a growth substrate under aerobic conditions.
Article in Applied and environmental microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 128 citations in OpenAlex.
- PFAS Biodegradation and the Constraints of Thermodynamics.Microbial biotechnology · 2025Article
- The biochemical mechanisms of plastic biodegradation.FEMS microbiology reviews · 2024Review
- Microbiome Composition and Dynamics of a Reductive/Oxidative Bioelectrochemical System for Perchloroethylene Removal: Effect of the Feeding Composition.Frontiers in microbiology · 2022Article
- Microbial and Enzymatic Degradation of Synthetic Plastics.Frontiers in microbiology · 2020Review
- Microbial degradation of chloroethenes: a review.Environmental science and pollution research international · 2017Review
- Nocardioides, Sediminibacterium, Aquabacterium, Variovorax, and Pseudomonas linked to carbon uptake during aerobic vinyl chloride biodegradation.Environmental science and pollution research international · 2016Article
- Kinetics of 1,2-dichloroethane and 1,2-dibromoethane biodegradation in anaerobic enrichment cultures.Applied and environmental microbiology · 2013Article
- Genome Sequence of the ethene- and vinyl chloride-oxidizing actinomycete Nocardioides sp. strain JS614.Journal of bacteriology · 2011Article
- Association of missense mutations in epoxyalkane coenzyme M transferase with adaptation of Mycobacterium sp. strain JS623 to growth on vinyl chloride.Applied and environmental microbiology · 2010Article
- Identification of polypeptides expressed in response to vinyl chloride, ethene, and epoxyethane in Nocardioides sp. strain JS614 by using peptide mass fingerprinting.Applied and environmental microbiology · 2007Article
- Involvement of coenzyme M during aerobic biodegradation of vinyl chloride and ethene by Pseudomonas putida strain AJ and Ochrobactrum sp. strain TD.Applied and environmental microbiology · 2006Article
- Coupling aggressive mass removal with microbial reductive dechlorination for remediation of DNAPL source zones: a review and assessment.Environmental health perspectives · 2005Review
- Involvement of linear plasmids in aerobic biodegradation of vinyl chloride.Applied and environmental microbiology · 2004Article
- Characterization of a highly enriched dehalococcoides-containing culture that grows on vinyl chloride and trichloroethene.Applied and environmental microbiology · 2004Article
- Biodegradation of chloromethane by Pseudomonas aeruginosa strain NB1 under nitrate-reducing and aerobic conditions.Applied and environmental microbiology · 2004Article
- Isopropanol and acetone induces vinyl chloride degradation in Rhodococcus rhodochrous.Journal of industrial microbiology & biotechnology · 2003Article
- Distribution of the coenzyme M pathway of epoxide metabolism among ethene- and vinyl chloride-degrading Mycobacterium strains.Applied and environmental microbiology · 2003Article
- Epoxyalkane: coenzyme M transferase in the ethene and vinyl chloride biodegradation pathways of mycobacterium strain JS60.Journal of bacteriology · 2003Article
- Phylogenetic and kinetic diversity of aerobic vinyl chloride-assimilating bacteria from contaminated sites.Applied and environmental microbiology · 2002Article
- Biodegradation of cis-dichloroethene as the sole carbon source by a beta-proteobacterium.Applied and environmental microbiology · 2002Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An aerobic enrichment culture was developed by using vinyl chloride (VC) as the sole organic carbon and electron donor source. VC concentrations as high as 7.3 mM were biodegraded without apparent inhibition. VC use did not occur when nitrate was provided as the electron acceptor. A gram-negative, rod-shaped, motile isolate was obtained from the enrichment culture and identified based on biochemical characteristics and the sequence of its 16S rRNA gene as Pseudomonas aeruginosa, designated strain MF1. The observed yield of MF1 when it was grown on VC was 0.20 mg of total suspended solids (TSS)/mg of VC. Ethene, acetate, glyoxylate, and glycolate also served as growth substrates, while ethane, chloroacetate, glycolaldehyde, and phenol did not. Stoichiometric release of chloride and minimal accumulation of soluble metabolites following VC consumption indicated that the predominant fate for VC is mineralization and incorporation into cell material. MF1 resumed consumption of VC after at least 24 days when none was provided, unlike various mycobacteria that lost their VC-degrading ability after brief periods in the absence of VC. When deprived of oxygen for 2.5 days, MF1 did not regain the ability to grow on VC, and a portion of the VC was transformed into VC-epoxide. Acetylene inhibited VC consumption by MF1, suggesting the involvement of a monooxygenase in the initial step of VC metabolism. The maximum specific VC utilization rate for MF1 was 0.41 micromol of VC/mg of TSS/day, the maximum specific growth rate was 0.0048/day, and the Monod half-saturation coefficient was 0.26 microM. A higher yield and faster kinetics occurred when MF1 grew on ethene. When grown on ethene, MF1 was able to switch to VC as a substrate without a lag. It therefore appears feasible to grow MF1 on a nontoxic substrate and then apply it to environments that do not exhibit a capacity for aerobic biodegradation of VC.
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