ArticleApplied and environmental microbiology2000
Quantitative determination of the biodegradable polymer Poly(beta-hydroxybutyrate) in a recombinant Escherichia coli strain by use of mid-infrared spectroscopy and multivariative statistics.
Article in Applied and environmental microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 151 citations in OpenAlex.
- State-of-the-art methods for quantifying microbial polyhydroxyalkanoates.Applied and environmental microbiology · 2025Review
- New Biodegradable Polyester-Polyurethane Biocompositions Enriched by Urea.Materials (Basel, Switzerland) · 2025Article
- Halomonas sp. MC140, a polyhydroxyalkanoate (PHA) producer isolated from the Arctic environment.Scientific reports · 2025Article
- Optimization and characterization of polyhydroxybutyrate produced by Halomonas meridiana using orange peel waste.BMC microbiology · 2025Article
- Nano-Infrared Detection and Identification of Bacteria at the Single-Cell Level.Analytical chemistry · 2025Article
- Uncovering novel polyhydroxyalkanoate biosynthesis genes and unique pathway in yeast hanseniaspora valbyensis for sustainable bioplastic production.Scientific reports · 2024Article
- Iron bioleaching and polymers accumulation by an extreme acidophilic bacterium.Archives of microbiology · 2024Article
- Physicochemical characterization of polyhydroxybutyrate (PHB) produced by the rare halophile Brachybacterium paraconglomeratum MTCC 13074.Microbial cell factories · 2024Article
- The Development and Characterisation of a P(3HB-Gels (Basel, Switzerland) · 2024Article
- Monitoring Phenotype Heterogeneity at the Single-Cell Level withinAnalytical chemistry · 2023Article
- Can Biomass Mastication Assist the Downstreaming of Polyhydroxyalkanoates Produced from Mixed Microbial Cultures?Molecules (Basel, Switzerland) · 2023Article
- Article
- Polyhydroxyalkanoate Decelerates the Release of Paclitaxel from Poly(lactic-co-glycolic acid) Nanoparticles.Pharmaceutics · 2022Article
- Advanced Waveguide Based LOC Biosensors: A Minireview.Sensors (Basel, Switzerland) · 2022Review
- In Situ Quantification of Polyhydroxybutyrate in Photobioreactor Cultivations ofBioengineering (Basel, Switzerland) · 2021Article
- Optimization and Characterization of PHA (SCL-SCL) Copolymer by Indigenous Bacillus thuringiensis A102 Strain for Biomedical Applications.Current microbiology · 2020Article
- Production, purification and evaluation of biodegradation potential of PHB depolymerase of Stenotrophomonas sp. RZS7.PloS one · 2020Article
- Bacterial biopolymer (polyhydroxyalkanoate) production from low-cost sustainable sources.MicrobiologyOpen · 2019Article
- Bioprocess Engineering Aspects of Sustainable Polyhydroxyalkanoate Production in Cyanobacteria.Bioengineering (Basel, Switzerland) · 2018Review
- Ultrasound-enhanced attenuated total reflection mid-infrared spectroscopy in-line probe: acquisition of cell spectra in a bioreactor.Analytical chemistry · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fourier transform infrared (FTIR) spectroscopy in combination with the partial least squares (PLS) multivariative statistical technique was used for quantitative analysis of the poly(beta-hydroxybutyrate) (PHB) contents of bacterial cells. A total of 237 replicate spectra from 34 samples were obtained together with gas chromatography-determined reference PHB contents. Using the PLS regression, we were able to relate the infrared spectra to the reference PHB contents, and the correlation coefficient between the measured and predicted values for the optimal model with a standard error of prediction of 1.49% PHB was 0.988. With this technique, there are no solvent requirements, sample preparation is minimal and simple, and analysis time is greatly reduced; our results demonstrate the potential of FTIR spectroscopy as an alternative to the conventional methods used for analysis of PHB in bacterial cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.