ArticleApplied and environmental microbiology2000
Identification of Mycobacterium ulcerans in the environment from regions in Southeast Australia in which it is endemic with sequence capture-PCR.
Article in Applied and environmental microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers, 1 of them a synthesis that pooled it.
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Who cites it
42 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Ecology and transmission of Buruli ulcer disease: a systematic review.PLoS neglected tropical diseases · 2010Pooled it
- Risk factors for Buruli ulcer disease in Ghana: A matched case-control study in four selected endemic districts of Eastern and Oti Regions.PLoS neglected tropical diseases · 2025Article
- Evaluation of an electricity-independent method for IS2404 Loop-mediated isothermal amplification (LAMP) diagnosis of Buruli ulcer in resource-limited settings.PLoS neglected tropical diseases · 2024Article
- Systematic review: Global host range, case fatality and detection rates ofJournal of clinical tuberculosis and other mycobacterial diseases · 2024Review
- Environmental risk factors associated with the presence of Mycobacterium ulcerans in Victoria, Australia.PloS one · 2022Article
- Leprosy in wild chimpanzees.Nature · 2021Article
- Understanding the transmission of Mycobacterium ulcerans: A step towards controlling Buruli ulcer.PLoS neglected tropical diseases · 2021Review
- Article
- Molecular detection of Mycobacterium ulcerans in the environment and its relationship with Buruli ulcer occurrence in Zio and Yoto districts of maritime region in Togo.PLoS neglected tropical diseases · 2018Article
- Importance of Local and Regional Scales in Shaping Mycobacterial Abundance in Freshwater Lakes.Microbial ecology · 2018Article
- Risk factors for Mycobacterium ulcerans infection (Buruli Ulcer) in Togo ─ a case-control study in Zio and Yoto districts of the maritime region.BMC infectious diseases · 2018Article
- Buruli Ulcer: a Review of the Current Knowledge.Current tropical medicine reports · 2018Review
- Environmental and Behavioral Drivers of Buruli Ulcer Disease in Selected Communities Along the Densu River Basin of Ghana: A Case-Control Study.The American journal of tropical medicine and hygiene · 2017Article
- Landscape and environmental influences on Mycobacterium ulcerans distribution among aquatic sites in Ghana.PloS one · 2017Article
- Experimental Survival of Mycobacterium ulcerans in Watery Soil, a Potential Source of Buruli Ulcer.The American journal of tropical medicine and hygiene · 2016Article
- Associations between Mycobacterium ulcerans and aquatic plant communities of West Africa: implications for Buruli ulcer disease.EcoHealth · 2014Article
- Genotyping Tools forMycobacterial diseases : tuberculosis & leprosy · 2014Article
- Analysis of the vaccine potential of plasmid DNA encoding nine mycolactone polyketide synthase domains in Mycobacterium ulcerans infected mice.PLoS neglected tropical diseases · 2014Article
- Topography and land cover of watersheds predicts the distribution of the environmental pathogen Mycobacterium ulcerans in aquatic insects.PLoS neglected tropical diseases · 2014Article
- Regulation of mycolactone, the Mycobacterium ulcerans toxin, depends on nutrient source.PLoS neglected tropical diseases · 2013Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We recently described the use of PCR to identify the environmental source of Mycobacterium ulcerans during an outbreak of ulcerative disease that occurred in a localized region of southeast Australia. The PCR used was based on amplification of the M. ulcerans-specific insertion sequence, IS2404. In this study we developed a new test that is a substantial improvement over the original PCR method in terms of sensitivity, reliability, and ease of use. In the new method magnetic bead sequence capture-PCR is used to detect two M. ulcerans sequences (IS2404 and IS2606) and total mycobacterial 16S ribosomal DNA. We used sequence capture-PCR to test water and plant material collected over a 12-month period during 1998 and 1999 from sites near the centers of two distinct foci of M. ulcerans infections. A golf course irrigation system in one area and a small shallow lake in another area repeatedly were PCR positive for M. ulcerans. Nearby sites and sites unrelated to the endemic areas were negative. Based on the PCR data, a most-probable-number method was used to estimate the concentration of M. ulcerans cells in positive samples from both regions. This procedure resulted in average concentrations of 0.5 cell per 100 ml of water and 40 cells per 100 g of detritus. Loss of the PCR signal coincided with a decrease in ulcerative disease in each area. These results provide further evidence that M. ulcerans may be transmitted from a point environmental source and demonstrate the utility of magnetic bead sequence capture-PCR for identification of nonculturable microbial pathogens in the environment.
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