ArticleOncogene2000
Substrate specificity of the p53-associated 3'-5' exonuclease.
Article in Oncogene, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 30 citations in OpenAlex.
- Review
- DNA damage tolerance pathway involving DNA polymerase ι and the tumor suppressor p53 regulates DNA replication fork progression.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Article
- Dissection of the sequence-specific DNA binding and exonuclease activities reveals a superactive yet apoptotically impaired mutant p53 protein.Cell cycle (Georgetown, Tex.) · 2009Article
- Exonucleolytic degradation of RNA by p53 protein in cytoplasm.Journal of molecular medicine (Berlin, Germany) · 2008Article
- WRN exonuclease activity is blocked by DNA termini harboring 3' obstructive groups.Mechanisms of ageing and development · 2007Article
- Excision of nucleoside analogs from DNA by p53 protein, a potential cellular mechanism of resistance to inhibitors of human immunodeficiency virus type 1 reverse transcriptase.Antimicrobial agents and chemotherapy · 2005Article
- Article
- P53 in cytoplasm may enhance the accuracy of DNA synthesis by human immunodeficiency virus type 1 reverse transcriptase.Oncogene · 2004Article
- p53-associated 3'-->5' exonuclease activity in nuclear and cytoplasmic compartments of cells.Oncogene · 2003Article
- DNA substrate dependence of p53-mediated regulation of double-strand break repair.Molecular and cellular biology · 2002Article
- DNA binding and 3'-5' exonuclease activity in the murine alternatively-spliced p53 protein.Oncogene · 2002Article
- Physical and functional interactions of the tumor suppressor protein p53 and DNA polymerase alpha-primase.Nucleic acids research · 2002Article
- p53 and recombination intermediates: role of tetramerization at DNA junctions in complex formation and exonucleolytic degradation.Oncogene · 2002Article
- PML NBs associate with the hMre11 complex and p53 at sites of irradiation induced DNA damage.Oncogene · 2002Article
- p53 enhances the fidelity of DNA synthesis by human immunodeficiency virus type 1 reverse transcriptase.Oncogene · 2001Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
p53 exhibits 3'-5' exonuclease activity and the significance of this biochemical function is currently not defined. In order to gain information about the potential role(s) of this exonuclease activity, recombinant and wild-type human p53 was examined for excision of nucleotides from defined synthetic DNA substrates. p53 removes nucleotides threefold faster from single-strand DNA than from DNA duplexes, exhibits a 1.5-fold preference for 3'-terminals of DNA that contain a single nucleotide mispair (mismatch) as compared to correctly paired DNA and efficiently excises nucleotides from 3'-ends of blunt and cohesive (staggered) DNA double-strand breaks. The p53 exonuclease is predominantly non-processive on DNA which is 17 nucleotides long (or shorter) and processive on the longer 30-mers. The processivity of nucleotide excision is decreased in the presence of 50 mM potassium phosphate and eliminated when full-length p53 is replaced with the core domain, comprised of amino acids 82-292. Photoaffinity labeling indicates that (1) p53 monomers, rather than dimers, bind to single-strand forms of these oligomers; (2) complexes between p53 and 30-mers are more stable than those formed with 17-mers. The stability of these complexes determines processivity during nucleotide removal and modulates the 3'-5' exonuclease activity of p53. The relevance of substrate specificity of the p53 exonuclease to DNA repair is discussed.
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