ArticleOncogene2000
Regulation of fibronectin matrix assembly by activated Ras in transformed cells.
Article in Oncogene, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
38 citing papers in PubMed, 70 citations in OpenAlex.
- Potential of Fibulin2 as a therapeutic target against cancer and as a diagnostic marker (Review).International journal of oncology · 2025Review
- Cell mediated remodeling of stiffness matched collagen and fibrin scaffolds.Scientific reports · 2022Article
- HIFα independent mechanisms in renal carcinoma cells modulate divergent outcomes in fibronectin assembly mediated by hypoxia and CoClScientific reports · 2020Article
- LRP1 is required for novobiocin-mediated fibronectin turnover.Scientific reports · 2018Article
- Dexamethasone-Mediated Upregulation of Calreticulin Inhibits Primary Human Glioblastoma Dispersal Ex Vivo.International journal of molecular sciences · 2018Article
- Stimulatory effects of advanced glycation endproducts (AGEs) on fibronectin matrix assembly.Matrix biology : journal of the International Society for Matrix Biology · 2017Article
- Diagnostic and Prognostic Relevance of Bone Marrow Microenvironment Components in Non Hodgkin’s Lymphoma Cases Before and After TherapyAsian Pacific journal of cancer prevention : APJCP · 2016Article
- The Phosphorylation and Distribution of Cortactin Downstream of Integrin α9β1 Affects Cancer Cell Behaviour.Scientific reports · 2016Article
- FAK, talin and PIPKIγ regulate endocytosed integrin activation to polarize focal adhesion assembly.Nature cell biology · 2016Article
- Studying early stages of fibronectin fibrillogenesis in living cells by atomic force microscopy.Molecular biology of the cell · 2015Article
- Dexamethasone Stiffens Trabecular Meshwork, Trabecular Meshwork Cells, and Matrix.Investigative ophthalmology & visual science · 2015Article
- Hsp90 binds directly to fibronectin (FN) and inhibition reduces the extracellular fibronectin matrix in breast cancer cells.PloS one · 2014Article
- Integrin αvβ3 and fibronectin upregulate Slug in cancer cells to promote clot invasion and metastasis.Cancer research · 2013Article
- Tumor cohesion and glioblastoma cell dispersal.Future oncology (London, England) · 2013Article
- RSK2 protein suppresses integrin activation and fibronectin matrix assembly and promotes cell migration.The Journal of biological chemistry · 2012Article
- At the leading edge of three-dimensional cell migration.Journal of cell science · 2012Review
- RGD-Dependent Epithelial Cell-Matrix Interactions in the Human Intestinal Crypt.Journal of signal transduction · 2012Article
- Plasma and cellular fibronectin: distinct and independent functions during tissue repair.Fibrogenesis & tissue repair · 2011Article
- KSHV regulation of fibulin-2 in Kaposi's sarcoma: implications for tumorigenesis.The American journal of pathology · 2011Article
- Fibronectins, their fibrillogenesis, and in vivo functions.Cold Spring Harbor perspectives in biology · 2011Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fibronectin extracellular matrix plays a critical role in the microenvironment of cells. Loss of this matrix frequently accompanies oncogenic transformation, allowing changes in cell growth, morphology, and tissue organization. The HT1080 human fibrosarcoma cell line is deficient in formation of fibronectin matrix fibrils but assembly can be induced by the glucocorticoid dexamethasone. Here we show that fibronectin assembly can also be restored by stimulation of alpha5beta1 integrin with activating antibody or with Mn2+ suggesting that integrin activity is reduced in these cells. While dexamethasone promoted actin stress fiber formation, actin filaments remained cortical following Mn2+ treatment showing that the dexamethasone effect is not due solely to cytoskeletal changes. HT1080 cells have one activated allele of N-ras and PD98059 inhibition of signaling from Ras through ERK increased fibronectin matrix accumulation. Conversely, the p38 MAP kinase inhibitor SB203580 blocked induction of matrix and increased ERK phosphorylation. Thus, two MAP kinase pathways contribute to the control of integrin-mediated fibronectin assembly. ERK activity and fibronectin assembly were linked in three different ras-transformed cell lines but not in SV40- or RSV-transformed cells indicating that oncogenic Ras uses a distinct mechanism to down-regulate cell-fibronectin interactions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.