ArticleRNA (New York, N.Y.)2000
Mature mRNAs accumulated in the nucleus are neither the molecules in transit to the cytoplasm nor constitute a stockpile for gene expression.
Article in RNA (New York, N.Y.), 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Seizure enhances SUMOylation and zinc-finger transcriptional repression in neuronal nuclei.iScience · 2023Article
- Sequences encoding C2H2 zinc fingers inhibit polyadenylation and mRNA export in human cells.Scientific reports · 2018Article
- Reciprocal antagonism between the netrin-1 receptor uncoordinated-phenotype-5A (UNC5A) and the hepatitis C virus.Oncogene · 2017Article
- Article
- Epidermal Growth Factor Receptor-Dependent Mutual Amplification between Netrin-1 and the Hepatitis C Virus.PLoS biology · 2016Article
- The rates of the major steps in the molecular mechanism of RNase H1-dependent antisense oligonucleotide induced degradation of RNA.Nucleic acids research · 2015Article
- Nuclear matrix protein Matrin 3 is a regulator of ZAP-mediated retroviral restriction.Retrovirology · 2015Article
- Regulation of mRNA export by the PI3 kinase/AKT signal transduction pathway.Molecular biology of the cell · 2013Article
- Regulation of H-ras splice variant expression by cross talk between the p53 and nonsense-mediated mRNA decay pathways.Molecular and cellular biology · 2007Article
- 3'-end formation signals modulate the association of genes with the nuclear periphery as well as mRNP dot formation.The EMBO journal · 2006Article
- In vivo BiFC analysis of Y14 and NXF1 mRNA export complexes: preferential localization within and around SC35 domains.The Journal of cell biology · 2006Article
- Splicing of human immunodeficiency virus RNA is position-dependent suggesting sequential removal of introns from the 5' end.Nucleic acids research · 2005Article
- Imaging native beta-actin mRNA in motile fibroblasts.Biophysical journal · 2004Article
- A novel function for Sam68: enhancement of HIV-1 RNA 3' end processing.RNA (New York, N.Y.) · 2004Article
- Human let-7 stem-loop precursors harbor features of RNase III cleavage products.Nucleic acids research · 2003Article
- In vivo kinetics of mRNA splicing and transport in mammalian cells.Molecular and cellular biology · 2002Article
- UBA1 and UBA2, two proteins that interact with UBP1, a multifunctional effector of pre-mRNA maturation in plants.Molecular and cellular biology · 2002Article
- Inhibition of human immunodeficiency virus type 1 Rev function by a dominant-negative mutant of Sam68 through sequestration of unspliced RNA at perinuclear bundles.Journal of virology · 2001Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In higher eukaryotes, the regulation of pre-mRNA processing is still poorly known. The accumulation of various mature mRNAs, which can be observed in the nuclei of mammalian cells, is suggestive of a regulatory role of transport. However, the significance of these nuclear mRNA is presently unknown. We have used a tetracycline-regulated promoter to investigate the dynamics of these pools of mRNAs upon arrest of transcription. We observed, for beta-globin and LT-alpha genes, a slow disappearance of these mRNA from the nucleus, with an apparent half-life that is similar to their cytoplasmic half-life. In view of these dynamics, these mRNA cannot simply be mature mRNAs in transit to the cytoplasm. They could be mRNAs retained in the nucleus, provided that the regulation of mRNA stability is comparable in the nucleus and the cytoplasm. But, because of their limited stability, these nuclear mRNAs cannot constitute a significant stock for gene expression. Alternatively, they could reflect a bidirectional transport of mRNA, that is, to and from the cytoplasm, which would provide a direct explanation for the similarity in both compartments of their half-life and poly(A) tail shortening over time.
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