ArticleMolecular and cellular biology2000
Analysis of the complex relationship between nuclear export and aryl hydrocarbon receptor-mediated gene regulation.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 65 citations in OpenAlex.
- AhR as a Common Denominator in Immunity and Inflammation in Chronic Lung Diseases: Molecular and Clinical Insights.Diseases (Basel, Switzerland) · 2026Review
- Aryl hydrocarbon receptor in mesenchymal stromal cells: new frontiers in AhR biology.The FEBS journal · 2021Review
- Plant Occurring Flavonoids as Modulators of the Aryl Hydrocarbon Receptor.Molecules (Basel, Switzerland) · 2021Review
- The Ah Receptor: Adaptive Metabolism, Ligand Diversity, and the Xenokine Model.Chemical research in toxicology · 2020Review
- Subcellular Localization Signals of bHLH-PAS Proteins: Their Significance, Current State of Knowledge and Future Perspectives.International journal of molecular sciences · 2019Review
- Wood smoke enhances cigarette smoke-induced inflammation by inducing the aryl hydrocarbon receptor repressor in airway epithelial cells.American journal of respiratory cell and molecular biology · 2015Article
- Aryl hydrocarbon receptor antagonism and its role in rheumatoid arthritis.Journal of experimental pharmacology · 2015Review
- Immunological Relevance of the Coevolution of IDO1 and AHR.Frontiers in immunology · 2014Review
- Pharmacological actions of multi-target-directed evodiamine.Molecules (Basel, Switzerland) · 2013Review
- Carnosol, a constituent of Zyflamend, inhibits aryl hydrocarbon receptor-mediated activation of CYP1A1 and CYP1B1 transcription and mutagenesis.Cancer prevention research (Philadelphia, Pa.) · 2012Article
- UVR exposure sensitizes keratinocytes to DNA adduct formation.Cancer prevention research (Philadelphia, Pa.) · 2009Article
- Timing is everything: consequences of transient and sustained AhR activity.Biochemical pharmacology · 2009Review
- Heat shock protein 90 inhibitors suppress aryl hydrocarbon receptor-mediated activation of CYP1A1 and CYP1B1 transcription and DNA adduct formation.Cancer prevention research (Philadelphia, Pa.) · 2008Article
- Styrene trimer may increase thyroid hormone levels via down-regulation of the aryl hydrocarbon receptor (AhR) target gene UDP-glucuronosyltransferase.Environmental health perspectives · 2008Article
- Regulation of the Drosophila hypoxia-inducible factor alpha Sima by CRM1-dependent nuclear export.Molecular and cellular biology · 2008Article
- Specific blockage of ligand-induced degradation of the Ah receptor by proteasome but not calpain inhibitors in cell culture lines from different species.Biochemical pharmacology · 2007Article
- Aryl hydrocarbon receptor activation by cAMP vs. dioxin: divergent signaling pathways.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- The aryl hydrocarbon receptor mediates degradation of estrogen receptor alpha through activation of proteasomes.Molecular and cellular biology · 2003Article
- A novel heterodimerization domain, CRM1, and 14-3-3 control subcellular localization of the MondoA-Mlx heterocomplex.Molecular and cellular biology · 2002Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The aryl hydrocarbon receptor (AHR) contains signals for both nuclear import and nuclear export (NES). The purpose of the studies in this report was to determine the relationship between the nuclear export of the AHR and AHR-mediated gene regulation. Blockage of nuclear export in HepG2 cells with leptomycin B (LMB) resulted in increased levels of AHR-AHR nuclear translocator (ARNT) complex in the nucleus and correlative reductions in agonist-stimulated AHR degradation. However, LMB exposure inhibited agonist-mediated induction of numerous AHR-responsive reporter genes by 75 to 89% and also inhibited induction of endogenous CYP1A1. LMB did not transform the AHR to a ligand binding species or affect activation by TCDD (2, 3,7,8-tetrachlorodibenzo-p-dioxin). Mutagenesis of leucines 66 and 71 of the putative AHR NES resulted in a protein with reduced function in dimerization to ARNT and binding to DNA, while alanine substitution at leucine 69 (AHR(A69)) resulted in an AHR that bound with ARNT and associated with DNA. AHR(A69) protein injected directly into the nuclei of E36 cells remained nuclear following 6 h of agonist stimulation. In transient-transfection assays, AHR(A69) accumulated within the nucleus was not degraded efficiently following agonist exposure. Finally, AHR(A69) supported induction of AHR-responsive reporter genes in an agonist-dependent manner. These findings show that it is possible to generate an AHR protein defective in nuclear export that is functional in agonist-mediated gene induction. This implies that the negative effect of LMB on agonist-mediated gene induction is independent of the nuclear export of the AHR.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.