ArticleMolecular and cellular biology2000
Separation of membrane trafficking and actin remodeling functions of ARF6 with an effector domain mutant.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed, 80 citations in OpenAlex.
- Rab35 governs apicobasal polarity through regulation of actin dynamics during sprouting angiogenesis.Nature communications · 2022Article
- The Recycling Endosome in Nerve Cell Development: One Rab to Rule Them All?Frontiers in cell and developmental biology · 2020Review
- ELMOD1 Stimulates ARF6-GTP Hydrolysis to Stabilize Apical Structures in Developing Vestibular Hair Cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
- C9ORF72 interaction with cofilin modulates actin dynamics in motor neurons.Nature neuroscience · 2016Article
- RLIP76 regulates Arf6-dependent cell spreading and migration by linking ARNO with activated R-Ras at recycling endosomes.Biochemical and biophysical research communications · 2015Article
- ADAP2 Is an Interferon Stimulated Gene That Restricts RNA Virus Entry.PLoS pathogens · 2015Article
- Endocytosis as a mode to regulate functional expression of two-pore domain potassium (K₂p) channels.Pflugers Archiv : European journal of physiology · 2015Review
- Regulators and Effectors of Arf GTPases in Neutrophils.Journal of immunology research · 2015Review
- Distinct recycling of active and inactive β1 integrins.Traffic (Copenhagen, Denmark) · 2012Article
- Secretion of soluble vascular endothelial growth factor receptor 1 (sVEGFR1/sFlt1) requires Arf1, Arf6, and Rab11 GTPases.PloS one · 2012Article
- Arf6 regulates AP-1B-dependent sorting in polarized epithelial cells.The Journal of cell biology · 2011Article
- Viral infection: Moving through complex and dynamic cell-membrane structures.Communicative & integrative biology · 2011Article
- HIV-1 requires Arf6-mediated membrane dynamics to efficiently enter and infect T lymphocytes.Molecular biology of the cell · 2011Article
- The structural basis of Arf effector specificity: the crystal structure of ARF6 in a complex with JIP4.The EMBO journal · 2009Article
- EFA6 facilitates the assembly of the tight junction by coordinating an Arf6-dependent and -independent pathway.The Journal of biological chemistry · 2008Article
- The PIX-GIT complex: a G protein signaling cassette in control of cell shape.Seminars in cell & developmental biology · 2008Review
- Trypanosoma brucei ARF1 plays a central role in endocytosis and golgi-lysosome trafficking.Molecular biology of the cell · 2007Article
- RLIP76 (RalBP1) is an R-Ras effector that mediates adhesion-dependent Rac activation and cell migration.The Journal of cell biology · 2006Article
- Extracellular signal-regulated kinase regulates clathrin-independent endosomal trafficking.Molecular biology of the cell · 2006Article
- A novel GTPase-activating protein for ARF6 directly interacts with clathrin and regulates clathrin-dependent endocytosis.Molecular biology of the cell · 2005Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The ADP-ribosylation factor 6 (ARF6) GTPase has a dual function in cells, regulating membrane traffic and organizing cortical actin. ARF6 activation is required for recycling of the endosomal membrane back to the plasma membrane (PM) and also for ruffling at the PM induced by Rac. Additionally, ARF6 at the PM induces the formation of actin-containing protrusions. To identify sequences in ARF6 that are necessary for these distinct functions, we examined the behavior of a chimeric protein of ARF1 and ARF6. The 1-6 chimera (with the amino half of ARF1 and the carboxyl half of ARF6) localized like ARF6 in HeLa cells and moved between the endosome and PM, but it did not form protrusions, an ARF6 effector function. Two residues in the amino-terminal half of ARF6, Q37 and S38, when substituted into the 1-6 chimera allowed protrusion formation, whereas removal of these residues from ARF6 resulted in an inability to form protrusions. Interestingly, expression of 1-6 in cells selectively inhibited protrusions induced by wild-type ARF6 but had no effect on ARF6-regulated membrane movement or Rac-induced ruffling. Thus, we have uncoupled two functions of ARF6, one involved in membrane trafficking, which is necessary for Rac ruffling, and another involved in protrusion formation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.