ArticleMolecular and cellular biology2000
Artificially recruited TATA-binding protein fails to remodel chromatin and does not activate three promoters that require chromatin remodeling.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 31 citations in OpenAlex.
- Dynamic epistasis analysis reveals how chromatin remodeling regulates transcriptional bursting.Nature structural & molecular biology · 2023Article
- Review
- BayesPI - a new model to study protein-DNA interactions: a case study of condition-specific protein binding parameters for Yeast transcription factors.BMC bioinformatics · 2009Article
- Chromatin-dependent transcription factor accessibility rather than nucleosome remodeling predominates during global transcriptional restructuring in Saccharomyces cerevisiae.Molecular biology of the cell · 2009Article
- Dispersed mutations in histone H3 that affect transcriptional repression and chromatin structure of the CHA1 promoter in Saccharomyces cerevisiae.Eukaryotic cell · 2008Article
- Artificial recruitment of mediator by the DNA-binding domain of Adr1 overcomes glucose repression of ADH2 expression.Molecular and cellular biology · 2008Article
- Promoter occupancy is a major determinant of chromatin remodeling enzyme requirements.Molecular and cellular biology · 2005Article
- Comparison of ABF1 and RAP1 in chromatin opening and transactivator potentiation in the budding yeast Saccharomyces cerevisiae.Molecular and cellular biology · 2004Article
- Genome-wide analysis of the relationship between transcriptional regulation by Rpd3p and the histone H3 and H4 amino termini in budding yeast.Molecular and cellular biology · 2004Article
- Specific TATAA and bZIP requirements suggest that HTLV-I Tax has transcriptional activity subsequent to the assembly of an initiation complex.Retrovirology · 2004Article
- Global and specific transcriptional repression by the histone H3 amino terminus in yeast.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- The different (sur)faces of Rap1p.Molecular genetics and genomics : MGG · 2003Review
- Protein-protein interaction map for yeast TFIID.Nucleic acids research · 2003Article
- The role of TFIIB-RNA polymerase II interaction in start site selection in yeast cells.Nucleic acids research · 2002Article
- Cloning and characterization of hELD/OSA1, a novel BRG1 interacting protein.The Biochemical journal · 2002Article
- GCN5 dependence of chromatin remodeling and transcriptional activation by the GAL4 and VP16 activation domains in budding yeast.Molecular and cellular biology · 2001Article
- Binding of TATA binding protein to a naturally positioned nucleosome is facilitated by histone acetylation.Molecular and cellular biology · 2001Article
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Transcriptional activators are believed to work in part by recruiting general transcription factors, such as TATA-binding protein (TBP) and the RNA polymerase II holoenzyme. Activation domains also contribute to remodeling of chromatin in vivo. To determine whether these two activities represent distinct functions of activation domains, we have examined transcriptional activation and chromatin remodeling accompanying artificial recruitment of TBP in yeast (Saccharomyces cerevisiae). We measured transcription of reporter genes with defined chromatin structure by artificial recruitment of TBP and found that a reporter gene whose TATA element was relatively accessible could be activated by artificially recruited TBP, whereas two promoters, GAL10 and CHA1, that have accessible activator binding sites, but nucleosomal TATA elements, could not. A third reporter gene containing the HIS4 promoter could be activated by GAL4-TBP only when a RAP1 binding site was present, although RAP1 alone could not activate the reporter, suggesting that RAP1 was needed to open the chromatin structure to allow activation. Consistent with this interpretation, artificially recruited TBP was unable to perturb nucleosome positioning via a nucleosomal binding site, in contrast to a true activator such as GAL4, or to perturb the TATA-containing nucleosome at the CHA1 promoter. Finally, we show that activation of the GAL10 promoter by GAL4, which requires chromatin remodeling, can occur even in swi gcn5 yeast, implying that remodeling pathways independent of GCN5, the SWI-SNF complex, and TFIID can operate during transcriptional activation in vivo.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.