ArticleMedical & biological engineering & computing2000
Spreading and motility of human glioblastoma cells on sheets of silicone rubber depend on substratum compliance.
Article in Medical & biological engineering & computing, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 50 citations in OpenAlex.
- Biomimetic Hydrogels in the Study of Cancer Mechanobiology: Overview, Biomedical Applications, and Future Perspectives.Gels (Basel, Switzerland) · 2022Review
- Strategies for developing complex multi-component in vitro tumor models: Highlights in glioblastoma.Advanced drug delivery reviews · 2022Review
- Atomic Force Microscope Nanoindentation Analysis of Diffuse Astrocytic Tumor Elasticity: Relation with Tumor Histopathology.Cancers · 2021Article
- Dissecting and rebuilding the glioblastoma microenvironment with engineered materials.Nature reviews. Materials · 2019Article
- The mechanical and pharmacological regulation of glioblastoma cell migration in 3D matrices.Journal of cellular physiology · 2019Article
- Effects of Liposomes Contained in Thermosensitive Hydrogels as Biomaterials Useful in Neural Tissue Engineering.Materials (Basel, Switzerland) · 2017Article
- Integrating the glioblastoma microenvironment into engineered experimental models.Future science OA · 2017Review
- Toward 3D biomimetic models to understand the behavior of glioblastoma multiforme cells.Tissue engineering. Part B, Reviews · 2014Review
- Master equation-based analysis of a motor-clutch model for cell traction force.Cellular and molecular bioengineering · 2013Article
- Determinants of maximal force transmission in a motor-clutch model of cell traction in a compliant microenvironment.Biophysical journal · 2013Article
- The alignment and fusion assembly of adipose-derived stem cells on mechanically patterned matrices.Biomaterials · 2012Article
- An in vitro correlation of mechanical forces and metastatic capacity.Physical biology · 2011Article
- Vascular smooth muscle cell durotaxis depends on substrate stiffness gradient strength.Biophysical journal · 2009Article
- The influence of hydrogel modulus on the proliferation and differentiation of encapsulated neural stem cells.Biomaterials · 2009Article
- The mechanical rigidity of the extracellular matrix regulates the structure, motility, and proliferation of glioma cells.Cancer research · 2009Article
- Substrate modulus directs neural stem cell behavior.Biophysical journal · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although there is a substantial quantity of experimental data examining the effects of adhesion on the morphology and migration of tissue cells, little attention has been focused on how changes in substratum mechanical properties affect these cellular behaviours. To determine whether the ability of a substratum mechanically to support traction influences cell morphology and motility, measurements are taken of the spreading, the fraction of a population with pseudopodia, the number of pseudopodia and the translocation of human SNB-19 glioblastoma cells cultured on films of poly(methylphenyl)siloxane possessing a range of mechanical compliances. Cells cultured on these films generate deformations (i.e. 'wrinkles') that are used as a basis to estimate effective substratum compliances. The average projected cell area decreases by over 60%, with a two-orders-of-magnitude increase in compliance. Time-lapse videomicroscopy reveals that cell migration also decreases with increasing compliance: the average cell speed decreases from approximately 8 microns h-1 on the most rigid substrata to 1.2 microns h-1 on the most compliant substrata examined. Changes in compliance do not alter mean directional persistence time. These results are interpreted in terms of the predictions of mathematical models for the effects of substratum compliance on motility.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.