ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2000
Depletion of a microtubule-associated motor protein induces the loss of dendritic identity.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 115 citations in OpenAlex.
- Active transport by cytoplasmic dynein sustains dendritic MAP2 localization in developing neurons.The Journal of biological chemistry · 2026Article
- Kinetochore proteins control microtubule dynamics in postmitotic neurons to regulate the formation of dendritic spines.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Reduced Expression of Selected Exosomal MicroRNAs Is Associated with Poor Outcomes in Patients with Acute Stroke Receiving Reperfusion Therapy-Preliminary Study.International journal of molecular sciences · 2025Article
- Deciphering the Tubulin Language: Molecular Determinants and Readout Mechanisms of the Tubulin Code in Neurons.International journal of molecular sciences · 2023Review
- An alternative splice isoform of mouse CDK5RAP2 induced cytoplasmic microtubule nucleation.IBRO neuroscience reports · 2022Article
- Article
- TIAM-1 differentially regulates dendritic and axonal microtubule organization in patterning neuronal development through its multiple domains.PLoS genetics · 2022Article
- An in vitro model of neuronal ensembles.Nature communications · 2022Article
- Microtubule polarity is instructive for many aspects of neuronal polarity.Developmental biology · 2022Article
- Principles of microtubule polarity in linear cells.Developmental biology · 2022Review
- Article
- Trim9 and Klp61F promote polymerization of new dendritic microtubules along parallel microtubules.Journal of cell science · 2021Article
- Mini-review: Microtubule sliding in neurons.Neuroscience letters · 2021Review
- Review
- Microtubule Dysfunction: A Common Feature of Neurodegenerative Diseases.International journal of molecular sciences · 2020Review
- Review
- Ser/Thr kinase Trc controls neurite outgrowth ineLife · 2020Article
- Patronin-mediated minus end growth is required for dendritic microtubule polarity.The Journal of cell biology · 2019Article
- Reduced presynaptic vesicle stores mediate cellular and network plasticity defects in an early-stage mouse model of Alzheimer's disease.Molecular neurodegeneration · 2019Article
- Polarity Sorting of Microtubules in the Axon.Trends in neurosciences · 2018Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dendrites are short stout tapering processes that are rich in ribosomes and Golgi elements, whereas axons are long thin processes of uniform diameter that are deficient in these organelles. It has been hypothesized that the unique morphological and compositional features of axons and dendrites result from their distinct patterns of microtubule polarity orientation. The microtubules within axons are uniformly oriented with their plus ends distal to the cell body, whereas microtubules within dendrites are nonuniformly oriented. The minus-end-distal microtubules are thought to arise via their specific transport into dendrites by the motor protein known as CHO1/MKLP1. According to this model, CHO1/MKLP1 transports microtubules with their minus ends leading into dendrites by generating forces against the plus-end-distal microtubules, thus creating drag on the plus-end-distal microtubules. Here we show that depletion of CHO1/MKLP1 from cultured neurons causes a rapid redistribution of microtubules within dendrites such that minus-end-distal microtubules are chased back to the cell body while plus-end-distal microtubules are redistributed forward. The dendrite grows significantly longer and thinner, loses its taper, and acquires a progressively more axon-like organelle composition. These results suggest that the forces generated by CHO1/MKLP1 are necessary for maintaining the minus-end-distal microtubules in the dendrite, for antagonizing the anterograde transport of the plus-end-distal microtubules, and for sustaining a pattern of microtubule organization necessary for the maintenance of dendritic morphology and composition. Thus, we would conclude that dendritic identity is dependent on forces generated by CHO1/MKLP1.
Indexed as
Identifiers
10908619PMC6772545W1583142746What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.