ArticleThe Journal of cell biology2000
Microtubules remodel actomyosin networks in Xenopus egg extracts via two mechanisms of F-actin transport.
Article in The Journal of cell biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
41 citing papers in PubMed, 104 citations in OpenAlex.
- Collective dynamics of actin and microtubule and its crosstalk mediated by FHDC1.Frontiers in cell and developmental biology · 2023Article
- Generation of stable microtubule superstructures by binding of peptide-fused tetrameric proteins to inside and outside.Science advances · 2022Article
- Review
- Article
- A highly dynamic F-actin network regulates transport and recycling of micronemes in Toxoplasma gondii vacuoles.Nature communications · 2019Article
- Models of convergent extension during morphogenesis.Wiley interdisciplinary reviews. Developmental biology · 2018Review
- Article
- Myosin Va plays essential roles in maintaining normal mitosis, enhancing tumor cell motility and viability.Oncotarget · 2017Article
- Article
- Nuclear deformability and telomere dynamics are regulated by cell geometric constraints.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Spindle Assembly and Chromosome Segregation Requires Central Spindle Proteins in Drosophila Oocytes.Genetics · 2016Article
- Microtubule nucleation remote from centrosomes may explain how asters span large cells.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- The actin-microtubule cross-linking activity of Drosophila Short stop is regulated by intramolecular inhibition.Molecular biology of the cell · 2013Article
- Confinement induces actin flow in a meiotic cytoplasm.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Aster migration determines the length scale of nuclear separation in the Drosophila syncytial embryo.The Journal of cell biology · 2012Article
- Differential interactions of the formins INF2, mDia1, and mDia2 with microtubules.Molecular biology of the cell · 2011Article
- Deficiency of the cytoskeletal protein SPECC1L leads to oblique facial clefting.American journal of human genetics · 2011Article
- Actin behavior in bulk cytoplasm is cell cycle regulated in early vertebrate embryos.Journal of cell science · 2011Article
- Drosophila katanin is a microtubule depolymerase that regulates cortical-microtubule plus-end interactions and cell migration.Nature cell biology · 2011Article
- Cytoskeletal dynamics and spermatogenesis.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2010Review
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Interactions between microtubules and filamentous actin (F-actin) are crucial for many cellular processes, including cell locomotion and cytokinesis, but are poorly understood. To define the basic principles governing microtubule/F-actin interactions, we used dual-wavelength digital fluorescence and fluorescent speckle microscopy to analyze microtubules and F-actin labeled with spectrally distinct fluorophores in interphase Xenopus egg extracts. In the absence of microtubules, networks of F-actin bundles zippered together or exhibited serpentine gliding along the coverslip. When microtubules were nucleated from Xenopus sperm centrosomes, they were released and translocated away from the aster center. In the presence of microtubules, F-actin exhibited two distinct, microtubule-dependent motilities: rapid ( approximately 250-300 nm/s) jerking and slow ( approximately 50 nm/s), straight gliding. Microtubules remodeled the F-actin network, as F-actin jerking caused centrifugal clearing of F-actin from around aster centers. F-actin jerking occurred when F-actin bound to motile microtubules powered by cytoplasmic dynein. F-actin straight gliding occurred when F-actin bundles translocated along the microtubule lattice. These interactions required Xenopus cytosolic factors. Localization of myosin-II to F-actin suggested it may power F-actin zippering, while localization of myosin-V on microtubules suggested it could mediate interactions between microtubules and F-actin. We examine current models for cytokinesis and cell motility in light of these findings.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.