ArticleNucleic acids research2000
Requirement for the SRS2 DNA helicase gene in non-homologous end joining in yeast.
Article in Nucleic acids research, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 57 citations in OpenAlex.
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- Pro-recombination Role of Srs2 Protein Requires SUMO (Small Ubiquitin-like Modifier) but Is Independent of PCNA (Proliferating Cell Nuclear Antigen) Interaction.The Journal of biological chemistry · 2016Article
- New vectors for simple and streamlined CRISPR-Cas9 genome editing in Saccharomyces cerevisiae.Yeast (Chichester, England) · 2015Article
- Requirement of POL3 and POL4 on non-homologous and microhomology-mediated end joining in rad50/xrs2 mutants of Saccharomyces cerevisiae.Mutagenesis · 2015Article
- Hyperactive Cdc2 kinase interferes with the response to broken replication forks by trapping S.pombe Crb2 in its mitotic T215 phosphorylated state.Nucleic acids research · 2014Article
- Is non-homologous end-joining really an inherently error-prone process?PLoS genetics · 2014Review
- Putative antirecombinase Srs2 DNA helicase promotes noncrossover homologous recombination avoiding loss of heterozygosity.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Differential requirement for SUB1 in chromosomal and plasmid double-strand DNA break repair.PloS one · 2013Article
- From yeast to mammals: recent advances in genetic control of homologous recombination.DNA repair · 2012Review
- Homologous recombination via synthesis-dependent strand annealing in yeast requires the Irc20 and Srs2 DNA helicases.Genetics · 2012Article
- End-processing during non-homologous end-joining: a role for exonuclease 1.Nucleic acids research · 2011Article
- Nej1 recruits the Srs2 helicase to DNA double-strand breaks and supports repair by a single-strand annealing-like mechanism.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- C. elegans SIR-2.1 translocation is linked to a proapoptotic pathway parallel to cep-1/p53 during DNA damage-induced apoptosis.Genes & development · 2008Article
- Nucleoporins prevent DNA damage accumulation by modulating Ulp1-dependent sumoylation processes.Molecular biology of the cell · 2007Article
- Telomere tethering at the nuclear periphery is essential for efficient DNA double strand break repair in subtelomeric region.The Journal of cell biology · 2006Article
- Rad51-dependent DNA structures accumulate at damaged replication forks in sgs1 mutants defective in the yeast ortholog of BLM RecQ helicase.Genes & development · 2005Article
- The RING finger ATPase Rad5p of Saccharomyces cerevisiae contributes to DNA double-strand break repair in a ubiquitin-independent manner.Nucleic acids research · 2005Article
- Saccharomyces cerevisiae Srs2 DNA helicase selectively blocks expansions of trinucleotide repeats.Molecular and cellular biology · 2004Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Mitotic cells experience double-strand breaks (DSBs) from both exogenous and endogenous sources. Since unrepaired DSBs can result in genome rearrangements or cell death, cells mobilize multiple pathways to repair the DNA damage. In the yeast Saccharomyces cerevisiae, mitotic cells preferentially use a homologous recombination repair pathway. However, when no significant homology to the DSB ends is available, cells utilize a repair process called non-homologous end joining (NHEJ), which can join ends with no homology through resection to uncover microhomologies of a few nucleotides. Although components of the homologous recombination repair system are also involved in NHEJ, the rejoining does not involve all of the homologous recombination repair genes. The SRS2 DNA helicase has been shown to be required for DSB repair when the homologous single-stranded regions are short. Here it is shown that SRS2 is also required for NHEJ, regardless of the cell mating type. Efficient NHEJ of sticky ends requires the Ku70 and Ku80 proteins and the silencing genes SIR2, SIR3 and SIR4. However, NHEJ of blunt ends, while very inefficient, is not further reduced by mutations in YKU70, SIR2, SIR3, SIR4 or SRS2, suggesting that this rejoining process occurs by a different mechanism.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.