ArticleLipids2000
Characterization of inositol phospholipids and identification of a mastoparan-induced polyphosphoinositide response in Tetrahymena pyriformis.
Article in Lipids, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
7 citing papers in PubMed, 21 citations in OpenAlex.
- Phospholipases Dα and δ are involved in local and systemic wound responses of cotton (Biochemistry and biophysics reports · 2017Article
- Article
- Conservation and innovation in Tetrahymena membrane traffic: proteins, lipids, and compartments.Methods in cell biology · 2012Review
- Emerging roles of phosphoinositide-specific phospholipases C in the ciliates Tetrahymena and Paramecium.Communicative & integrative biology · 2011Article
- Biochemical and genetic evidence for the presence of multiple phosphatidylinositol- and phosphatidylinositol 4,5-bisphosphate-specific phospholipases C in Tetrahymena.Eukaryotic cell · 2011Article
- Study of the Effect of Methyl Jasmonate Concentration on Aflatoxin B(1) Biosynthesis by Aspergillus parasiticus in Yeast Extract Sucrose Medium.International journal of microbiology · 2009Article
- GTP avoidance in Tetrahymena thermophila requires tyrosine kinase activity, intracellular calcium, NOS, and guanylyl cyclase.Purinergic signalling · 2008Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The unicellular eukaryote Tetrahymena is a popular model for the study of lipid metabolism. Less attention, however, has been given to the inositol phospholipids of the cell, although it is known that this class of lipids plays an important role in eukaryotic cell signaling. Tetrahymena pyriformis phosphatidylinositol was isolated, purified, and characterized by proton nuclear magnetic resonance analysis and [2-(3)H]myoinositol labeling. Labeling was also used for polyphosphoinositide (phosphatidylinositol phosphate and phosphatidylinositol bisphosphate) identification. Tetrahymena inositol phospholipids were found to belong to the diacylglycerol group, although major Tetrahymena phospholipids, phosphatidylcholine and aminoethylphosphonoglycerides, have been found to be mainly alkylacylglyceroderivatives. Further characterization of Tetrahymena phosphatidylinositol by gas chromatographic analysis indicated that 80% of fatty acids were myristic acid and palmitic acid. This is also in contrast to the fatty acid profile of Tetrahymena phosphatidylcholine and phosphatidylethanolamine, with respect both to the fatty acid length and degree of unsaturation, and may indicate that specific diacylglycerol species are connected with the phosphatidylinositol metabolism in this cell. Treatment of [3H]inositol-labeled Tetrahymena cells with mastoparan, a G-protein-activating peptide, induced changes in the polyphosphoinositide levels, suggesting that inositol phospholipids may form in Tetrahymena a functional signaling system similar to that of higher eukaryotes. Addition of 10 microM mastoparan resulted in a rapid and transient increase in [3H]phosphatidylinositol phosphate followed by a decrease in [3H]phosphatidylinositol bisphosphate. Similar changes in lipids have been reported when phosphoinositide-phospholipase C pathway is activated in both animal and plant cells.
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