ArticleJournal of virology2000
Essential and dispensable virus-encoded replication elements revealed by efforts To develop hypoviruses as gene expression vectors.
Article in Journal of virology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 61 citations in OpenAlex.
- Article
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- A Mycovirus VIGS Vector Confers Hypovirulence to a Plant Pathogenic Fungus to Control Wheat FHB.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Facilitative and synergistic interactions between fungal and plant viruses.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Article
- Gene silencing and gene expression in phytopathogenic fungi using a plant virus vector.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Article
- Something new to explore: Plant viruses infecting and inducing gene silencing in filamentous fungi.Mobile genetic elements · 2014Article
- Variations in hypovirus interactions with the fungal-host RNA-silencing antiviral-defense response.Journal of virology · 2012Article
- Acquiring transgenic tobacco plants with insect resistance and glyphosate tolerance by fusion gene transformation.Plant cell reports · 2012Article
- Conserved and variable structural elements in the 5' untranslated region of two hypoviruses from the filamentous fungus Cryphonectria parasitica.Virus research · 2011Article
- Mycoviruses, RNA silencing, and viral RNA recombination.Advances in virus research · 2011Article
- A single Argonaute gene is required for induction of RNA silencing antiviral defense and promotes viral RNA recombination.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Controlled gene expression in the plant pathogen Cryphonectria parasitica by use of a copper-responsive element.Applied and environmental microbiology · 2009Article
- Coupled termination/reinitiation for translation of the downstream open reading frame B of the prototypic hypovirus CHV1-EP713.Nucleic acids research · 2009Article
- Intragenic rearrangements of a mycoreovirus induced by the multifunctional protein p29 encoded by the prototypic hypovirus CHV1-EP713.RNA (New York, N.Y.) · 2008Article
- A host dicer is required for defective viral RNA production and recombinant virus vector RNA instability for a positive sense RNA virus.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Hypovirus papain-like protease p48 is required for initiation but not for maintenance of virus RNA propagation in the chestnut blight fungus Cryphonectria parasitica.Journal of virology · 2008Article
- A host factor involved in hypovirus symptom expression in the chestnut blight fungus, Cryphonectria parasitica.Journal of virology · 2008Article
- Genome sequence, full-length infectious cDNA clone, and mapping of viral double-stranded RNA accumulation determinant of hypovirus CHV1-EP721.Journal of virology · 2007Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We have investigated whether hypoviruses, viral agents responsible for virulence attenuation (hypovirulence) of the chestnut blight fungus Cryphonectria parasitica, could serve as gene expression vectors. The infectious cDNA clone of the prototypic hypovirus CHV1-EP713 was modified to generate 20 different vector candidates. Although transient expression was achieved for a subset of vectors that contained the green fluorescent protein gene from Aequorea victoria, long-term expression (past day 8) was not observed for any vector construct. Analysis of viral RNAs recovered from transfected fungal colonies revealed that the foreign genes were readily deleted from the replicating virus, although small portions of foreign sequences were retained by some vectors after months of replication. However, the results of vector viability and progeny characterization provided unexpected new insights into essential and dispensable elements of hypovirus replication. The N-terminal portion (codons 1 to 24) of the 5'-proximal open reading frame (ORF), ORF A, was found to be required for virus replication, while the remaining 598 codons of this ORF were completely dispensable. Substantial alterations were tolerated in the pentanucleotide UAAUG that contains the ORF A termination codon and the overlapping putative initiation codon of the second of the two hypovirus ORFs, ORF B. Replication competence was maintained following either a frameshift mutation that caused a two-codon extension of ORF A or a modification that produced a single-ORF genomic organization. These results are discussed in terms of determinants of hypovirus replication, the potential utility of hypoviruses as gene expression vectors, and possible mechanisms by which hypoviruses recognize and delete foreign sequences.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.