ArticleJournal of virology2000
Assembly of retrovirus capsid-nucleocapsid proteins in the presence of membranes or RNA.
Article in Journal of virology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 34 citations in OpenAlex.
- The cooperative function of arginine residues in the Prototype Foamy Virus Gag C-terminus mediates viral and cellular RNA encapsidation.Retrovirology · 2014Article
- Analysis of small molecule ligands targeting the HIV-1 matrix protein-RNA binding site.The Journal of biological chemistry · 2013Article
- Abrogation of contaminating RNA activity in HIV-1 Gag VLPs.Virology journal · 2011Article
- HIV-1 matrix protein binding to RNA.Journal of molecular biology · 2011Article
- Ordered assembly of murine leukemia virus capsid protein on lipid nanotubes directs specific binding by the restriction factor, Fv1.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Implications of the nucleocapsid and the microenvironment in retroviral reverse transcription.Viruses · 2010Article
- Analysis of human immunodeficiency virus type 1 matrix binding to membranes and nucleic acids.Journal of virology · 2009Article
- Human immunodeficiency virus type 1 matrix protein assembles on membranes as a hexamer.Journal of virology · 2007Article
- Supramolecular architecture of severe acute respiratory syndrome coronavirus revealed by electron cryomicroscopy.Journal of virology · 2006Article
- Molecular characterization of the HIV-1 gag nucleocapsid gene associated with vertical transmission.Retrovirology · 2006Article
- Hepatitis C virus core protein is a dimeric alpha-helical protein exhibiting membrane protein features.Journal of virology · 2005Article
- Investigation by atomic force microscopy of the structure of Ty3 retrotransposon particles.Journal of virology · 2005Article
- Three-dimensional structure of the M-MuLV CA protein on a lipid monolayer: a general model for retroviral capsid assembly.The EMBO journal · 2003Article
- Nucleic acid-independent retrovirus assembly can be driven by dimerization.Journal of virology · 2002Article
- Rous sarcoma virus Gag protein-oligonucleotide interaction suggests a critical role for protein dimer formation in assembly.Journal of virology · 2002Article
- Molecular organization of Mason-Pfizer monkey virus capsids assembled from Gag polyprotein in Escherichia coli.Journal of virology · 2002Article
- RNA is a structural element in retrovirus particles.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Cell biology of retroviral RNA packaging.RNA biologyReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
Abstract
Retrovirus Gag precursor (PrGag) proteins direct the assembly of roughly spherical immature virus particles, while after proteolytic processing events, the Gag capsid (CA) and nucleocapsid (NC) domains condense on viral RNAs to form mature retrovirus core structures. To investigate the process of retroviral morphogenesis, we examined the properties of histidine-tagged (His-tagged) Moloney murine leukemia (M-MuLV) capsid plus nucleocapsid (CANC) (His-MoCANC) proteins in vitro. The His-MoCANC proteins bound RNA, possessed nucleic acid-annealing activities, and assembled into strand, circle (or sphere), and tube forms in the presence of RNA. Image analysis of electron micrographs revealed that tubes were formed by cage-like lattices of CANC proteins surrounding at least two different types of protein-free cage holes. By virtue of a His tag association with nickel-chelating lipids, His-MoCANC proteins also assembled into planar sheets on lipid monolayers, mimicking the membrane-associated immature PrGag protein forms. Membrane-bound His-MoCANC proteins organized into two-dimensional (2D) cage-like lattices that were closely related to the tube forms, and in the presence of both nickel-chelating lipids and RNAs, 2D lattice forms appeared similar to lattices assembled in the absence of RNA. Our observations are consistent with a M-MuLV morphogenesis model in which proteolytic processing of membrane-bound Gag proteins permits CA and NC domains to rearrange from an immature spherical structure to a condensed mature form while maintaining local protein-protein contacts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.