ArticleYeast (Chichester, England)2000
Genome-wide protein interaction screens reveal functional networks involving Sm-like proteins.
Article in Yeast (Chichester, England), 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 83 papers.
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Who cites it
83 citing papers in PubMed.
- Bioinformatic Analyses of the Ataxin-2 Family Since Algae Emphasize Its Small Isoforms, Large Chimerisms, and the Importance of Human Exon 1B as Target of Therapies to Prevent Neurodegeneration.International journal of molecular sciences · 2026Article
- MS-Based Proteomics Approach in Mapping Protein-Protein Interaction.Advances in experimental medicine and biology · 2026Review
- Swt21p Is Required for Nam8p-U1 snRNP Association and Efficient Pre-mRNA Splicing inInternational journal of molecular sciences · 2025Article
- Design of pseudosymmetric protein hetero-oligomers.Nature communications · 2024Article
- Eukaryotic mRNA decapping factors: molecular mechanisms and activity.The FEBS journal · 2023Review
- Article
- Eukaryotic mRNA Decapping Activation.Frontiers in genetics · 2022Review
- Yeast Nst1 is a novel component of P-bodies and is a specific suppressor of proteasome base assembly defects.Molecular biology of the cell · 2021Article
- The life of U6 small nuclear RNA, from cradle to grave.RNA (New York, N.Y.) · 2018Review
- Rapid isolation and single-molecule analysis of ribonucleoproteins from cell lysate by SNAP-SiMPull.RNA (New York, N.Y.) · 2015Article
- A yeast two-hybrid approach for probing protein-protein interactions at the centrosome.Methods in cell biology · 2015Article
- Yeast Edc3 targets RPS28B mRNA for decapping by binding to a 3' untranslated region decay-inducing regulatory element.Molecular and cellular biology · 2014Article
- The C-terminal domain from S. cerevisiae Pat1 displays two conserved regions involved in decapping factor recruitment.PloS one · 2014Article
- Proteins associated with SF3a60 in T. brucei.PloS one · 2014Article
- The role of disordered protein regions in the assembly of decapping complexes and RNP granules.Genes & development · 2013Review
- Identification of the Rps28 binding motif from yeast Edc3 involved in the autoregulatory feedback loop controlling RPS28B mRNA decay.Nucleic acids research · 2013Article
- Archaeal and eukaryotic homologs of Hfq: A structural and evolutionary perspective on Sm function.RNA biology · 2013Review
- Predicting the binding patterns of hub proteins: a study using yeast protein interaction networks.PloS one · 2013Article
- A direct interaction between DCP1 and XRN1 couples mRNA decapping to 5' exonucleolytic degradation.Nature structural & molecular biology · 2012Article
- A comparison and optimization of yeast two-hybrid systems.Methods (San Diego, Calif.) · 2012Article
23 more citing papers are in PubMed but not listed here.
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11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A set of seven structurally related Sm proteins forms the core of the snRNP particles containing the spliceosomal U1, U2, U4 and U5 snRNAs. A search of the genomic sequence of Saccharomyces cerevisiae has identified a number of open reading frames that potentially encode structurally similar proteins termed Lsm (Like Sm) proteins. With the aim of analysing all possible interactions between the Lsm proteins and any protein encoded in the yeast genome, we performed exhaustive and iterative genomic two-hybrid screens, starting with the Lsm proteins as baits. Indeed, extensive interactions amongst eight Lsm proteins were found that suggest the existence of a Lsm complex or complexes. These Lsm interactions apparently involve the conserved Sm domain that also mediates interactions between the Sm proteins. The screens also reveal functionally significant interactions with splicing factors, in particular with Prp4 and Prp24, compatible with genetic studies and with the reported association of Lsm proteins with spliceosomal U6 and U4/U6 particles. In addition, interactions with proteins involved in mRNA turnover, such as Mrt1, Dcp1, Dcp2 and Xrn1, point to roles for Lsm complexes in distinct RNA metabolic processes, that are confirmed in independent functional studies. These results provide compelling evidence that two-hybrid screens yield functionally meaningful information about protein-protein interactions and can suggest functions for uncharacterized proteins, especially when they are performed on a genome-wide scale.
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