ArticleProceedings of the National Academy of Sciences of the United States of America2000
High-frequency intrachromosomal gene conversion induced by triplex-forming oligonucleotides microinjected into mouse cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 93 citations in OpenAlex.
- Purine- and pyrimidine-triple-helix-forming oligonucleotides recognize qualitatively different target sites at the ribosomal DNA locus.RNA (New York, N.Y.) · 2018Article
- Tools to Study the Role of Architectural Protein HMGB1 in the Processing of Helix Distorting, Site-specific DNA Interstrand Crosslinks.Journal of visualized experiments : JoVE · 2016Article
- Therapeutic genome mutagenesis using synthetic donor DNA and triplex-forming molecules.Methods in molecular biology (Clifton, N.J.) · 2015Article
- Binding of novel 9-O-N-aryl/arylalkyl amino carbonyl methyl berberine analogs to poly(U)-poly(A)·poly(U) triplex and comparison to the duplex poly(A)-poly(U).Molecular biology reports · 2014Article
- Local delivery of gene-modifying triplex-forming molecules to the epidermis.The Journal of investigative dermatology · 2013Article
- Review
- Targeting and processing of site-specific DNA interstrand crosslinks.Environmental and molecular mutagenesis · 2010Review
- Targeted gene conversion induced by triplex-directed psoralen interstrand crosslinks in mammalian cells.Nucleic acids research · 2009Article
- Processing of triplex-directed psoralen DNA interstrand crosslinks by recombination mechanisms.Nucleic acids research · 2008Article
- Single-strand DNA-mediated targeted mutagenesis of genomic DNA in early mouse embryos is stimulated by Rad51/54 and by Ku70/86 inhibition.Gene therapy · 2008Article
- Third strand-mediated psoralen-induced correction of the sickle cell mutation on a plasmid transfected into COS-7 cells.Gene therapy · 2007Article
- The paperclip triplex: understanding the role of apex residues in tight turns.Biophysical journal · 2006Article
- Exploring the reasons for the large density of triplex-forming oligonucleotide target sequences in the human regulatory regions.BMC genomics · 2006Article
- Long homopurine*homopyrimidine sequences are characteristic of genes expressed in brain and the pseudoautosomal region.Nucleic acids research · 2006Article
- Triplex-induced recombination and repair in the pyrimidine motif.Nucleic acids research · 2005Article
- Naturally occurring H-DNA-forming sequences are mutagenic in mammalian cells.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Triplex-forming oligonucleotide target sequences in the human genome.Nucleic acids research · 2004Article
- Peptide conjugates for chromosomal gene targeting by triplex-forming oligonucleotides.Nucleic acids research · 2004Article
- Therapeutic modulation of endogenous gene function by agents with designed DNA-sequence specificities.Nucleic acids research · 2003Review
- Branched oligonucleotides induce in vivo gene conversion of a mutated EGFP reporter.Gene therapy · 2003Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
To test the ability of triple helix-forming oligonucleotides (TFOs) to promote recombination within chromosomal sites in mammalian cells, a mouse LTK(-) cell line was established carrying two mutant copies of the herpes simplex virus thymidine kinase (TK) gene as direct repeats in a single chromosomal locus. Recombination between these repeats can produce a functional TK gene and occurs at a spontaneous frequency of 4 x 10(-6) under standard culture conditions. When cells were microinjected with TFOs designed to bind to a 30-bp polypurine site situated between the two TK genes, recombination was observed at frequencies in the range of 1%, 2,500-fold above the background. Recombination was induced efficiently by injection of both psoralen-conjugated TFOs (followed by long-wave UVA light; 1. 2%) and unconjugated TFOs alone (1.0%). Control oligomers of scrambled sequence but identical base composition were ineffective, and no TFO-induced recombination was seen in a control LTK(-) cell line carrying an otherwise identical dual TK gene construct lacking the 30-bp polypurine target site. TFOs transfected with cationic lipids also induced recombinants in a highly sequence-specific manner but were less effective, with induced recombination frequencies of 6- to 7-fold over background. Examination of the TFO-induced recombinants by genomic Southern blotting revealed gene conversion events in which both TK genes were retained, but either the upstream (57%) or the downstream gene (43%) was corrected to wild type. These results suggest that, with efficient intracellular delivery, TFOs may be effective tools to promote site-specific recombination and targeted modification of chromosomal loci.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.