ArticleProceedings of the National Academy of Sciences of the United States of America2000
The spliceosomal snRNP core complex of Trypanosoma brucei: cloning and functional analysis reveals seven Sm protein constituents.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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30 citing papers in PubMed, 61 citations in OpenAlex.
- Sequences and proteins that influence mRNA processing in Trypanosoma brucei: Evolutionary conservation of SR-domain and PTB protein functions.PLoS neglected tropical diseases · 2022Article
- Molecular interaction between small nuclear ribonucleoprotein polypeptide G and heat shock protein 70.14: a microscale thermophoresis exposition towards developing anti-cancer drugs.American journal of translational research · 2022Article
- A nuclear enterprise: zooming in on nuclear organization and gene expression control in the African trypanosome.Parasitology · 2021Review
- Microscale thermophoresis analysis of the molecular interaction between small nuclear ribonucleoprotein polypeptide G and the RING finger domain of RBBP6 towards anti-cancer drug discovery.American journal of translational research · 2021Article
- The sole LSm complex inRNA (New York, N.Y.) · 2017Article
- Exosome secretion affects social motility in Trypanosoma brucei.PLoS pathogens · 2017Article
- The spliceosomal PRP19 complex of trypanosomes.Molecular microbiology · 2015Article
- Depletion of the SR-Related Protein TbRRM1 Leads to Cell Cycle Arrest and Apoptosis-Like Death in Trypanosoma brucei.PloS one · 2015Article
- Organization of Plasmodium falciparum spliceosomal core complex and role of arginine methylation in its assembly.Malaria journal · 2013Article
- Archaeal and eukaryotic homologs of Hfq: A structural and evolutionary perspective on Sm function.RNA biology · 2013Review
- Analysis of spliceosomal proteins in Trypanosomatids reveals novel functions in mRNA processing.The Journal of biological chemistry · 2010Article
- The pre-mRNA splicing machinery of trypanosomes: complex or simplified?Eukaryotic cell · 2010Review
- Essential role of a trypanosome U4-specific Sm core protein in small nuclear ribonucleoprotein assembly and splicing.Eukaryotic cell · 2010Article
- Special Sm core complex functions in assembly of the U2 small nuclear ribonucleoprotein of Trypanosoma brucei.Eukaryotic cell · 2009Article
- SMN-assisted assembly of snRNP-specific Sm cores in trypanosomes.Genes & development · 2009Article
- Spliceosomal proteomics in Trypanosoma brucei reveal new RNA splicing factors.Eukaryotic cell · 2009Article
- Evolutionary diversification of the Sm family of RNA-associated proteins.Molecular biology and evolution · 2008Review
- Characterization of the Trypanosoma brucei cap hypermethylase Tgs1.Molecular and biochemical parasitology · 2007Article
- Evolutionarily divergent type II protein arginine methyltransferase in Trypanosoma brucei.Eukaryotic cell · 2007Article
- Identification of novel snRNA-specific Sm proteins that bind selectively to U2 and U4 snRNAs in Trypanosoma brucei.RNA (New York, N.Y.) · 2007Article
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Each of the trypanosome small nuclear ribonucleoproteins (snRNPs) U2, U4/U6, and U5, as well as the spliced leader (SL) RNP, contains a core of common proteins, which we have previously identified. This core is unusual because it is not recognized by anti-Sm Abs and it associates with an Sm-related sequence in the trypanosome small nuclear RNAs (snRNAs). Using peptide sequences derived from affinity-purified U2 snRNP proteins, we have cloned cDNAs for five common proteins of 8.5, 10, 12.5, 14, and 15 kDa of Trypanosoma brucei and identified them as Sm proteins SmF (8.5 kDa), -E (10 kDa), -D1 (12.5 kDa), -G (14 kDa), and -D2 (15 kDa), respectively. Furthermore, we found the trypanosome SmB (T. brucei) and SmD3 (Trypanosoma cruzi) homologues through database searches, thus completing a set of seven canonical Sm proteins. Sequence comparisons of the trypanosome proteins revealed several deviations in highly conserved positions from the Sm consensus motif. We have identified a network of specific heterodimeric and -trimeric Sm protein interactions in vitro. These results are summarized in a model of the trypanosome Sm core, which argues for a strong conservation of the Sm particle structure. The conservation extends also to the functional level, because at least one trypanosome Sm protein, SmG, was able to specifically complement a corresponding mutation in yeast.
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