ArticleThe Journal of experimental medicine2000
A macrophage invasion mechanism for mycobacteria implicating the extracellular domain of CD43.
Article in The Journal of experimental medicine, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 57 citations in OpenAlex.
- The Sialomucin CD43 Drives Tuberculosis Immunopathology Across Different Host Genetic Backgrounds.Inflammation · 2026Article
- The important role of Perforin in protecting against Mycobacterium avium infection in mice.Scientific reports · 2025Article
- Risk assessment with gene expression markers in sepsis development.Cell reports. Medicine · 2024Article
- Negative Regulation of Autophagy during Macrophage Infection byInternational journal of molecular sciences · 2024Article
- Targeting CD43 optimizes cancer immunotherapy through reinvigorating antitumor immune response in colorectal cancer.Cellular oncology (Dordrecht, Netherlands) · 2023Article
- TNFR1 Mediated Apoptosis Is Protective againstMicroorganisms · 2023Article
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- Interaction of the CD43 Sialomucin with the Mycobacterium tuberculosis Cpn60.2 Chaperonin Leads to Tumor Necrosis Factor Alpha Production.Infection and immunity · 2017Article
- Analysis of Differentially Expressed Proteins inBioMed research international · 2017Article
- Activation of the Wnt Pathway byFrontiers in immunology · 2017Review
- Identification of novel genetic variations affecting osteoarthritis patients.BMC medical genetics · 2016Article
- Common polymorphisms in the CD43 gene region are associated with tuberculosis disease and mortality.American journal of respiratory cell and molecular biology · 2015Article
- Expression of leukosialin (CD43) defines a major intrahepatic T cell subset associated with protective responses in visceral leishmaniasis.Parasites & vectors · 2015Article
- Bacterial virulence in the moonlight: multitasking bacterial moonlighting proteins are virulence determinants in infectious disease.Infection and immunity · 2011Review
- Factors associated with pastoral community knowledge and occurrence of mycobacterial infections in human-animal interface areas of Nakasongola and Mubende districts, Uganda.BMC public health · 2010Article
- Impaired host defence against Mycobacterium avium in mice with chronic granulomatous disease.Clinical and experimental immunology · 2010Article
- Non-opsonic recognition of Mycobacterium tuberculosis by phagocytes.Journal of innate immunity · 2009Review
- Mycobacterium tuberculosis Rv2536 protein implicated in specific binding to human cell lines.Protein science : a publication of the Protein Society · 2005Article
- CD43 regulation of T cell activation is not through steric inhibition of T cell-APC interactions but through an intracellular mechanism.The Journal of experimental medicine · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 6 institutions in 1 country.
Funding
Abstract
We studied the role of CD43 (leukosialin/sialophorin), the negatively charged sialoglycoprotein of leukocytes, in the binding of mycobacteria to host cells. CD43-transfected HeLa cells bound Mycobacterium avium, but not Salmonella typhimurium or Shigella flexneri. Quantitative bacteriology showed that macrophages (M(phi)) from wild-type mice (CD43(+/+)) bound M. avium, Mycobacterium bovis (bacillus Calmette-Guérin), and Mycobacterium tuberculosis (strain H37Rv), whereas M(phi) from CD43 knockout mice (CD43(-/)-) did not. Fluorescence microscopy demonstrated that the associated M. avium had been ingested by the CD43(+/+) M(phi). The inability of CD43(-/)- M(phi) to bind M. avium could be restored by addition of galactoglycoprotein (Galgp), the extracellular mucin portion of CD43. The effect of Galgp is not due to opsonization of the bacteria, but required its interaction with the M(phi) other mucins had no effect. CD43 expression by the M(phi) was also required for optimal induction by M. avium of tumor necrosis factor (TNF)-alpha production, which likewise could be reconstituted by Galgp. In contrast, interleukin (IL)-10 production by M. avium-infected M(phi) was CD43 independent, demonstrating discordant regulation of TNF-alpha and IL-10. These findings describe a novel role of CD43 in promoting stable interaction of mycobacteria with receptors on the M(phi) enabling the cells to respond specifically with TNF-alpha production.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.