ArticleInfection and immunity2000
Identification of saliva-regulated genes of Streptococcus gordonii DL1 by differential display using random arbitrarily primed PCR.
Article in Infection and immunity, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 58 citations in OpenAlex.
- Comparison of periodontitis-associated oral biofilm formation under dynamic and static conditions.Journal of periodontal & implant science · 2017Article
- Mutation of the Thiol-Disulfide Oxidoreductase SdbA Activates the CiaRH Two-Component System, Leading to Bacteriocin Expression Shutdown in Streptococcus gordonii.Journal of bacteriology · 2016Article
- Article
- Article
- Intercellular communications in multispecies oral microbial communities.Frontiers in microbiology · 2014Review
- Autoinducer-2 influences interactions amongst pioneer colonizing streptococci in oral biofilms.Microbiology (Reading, England) · 2012Article
- Group A streptococcus adheres to pharyngeal epithelial cells with salivary proline-rich proteins via GrpE chaperone protein.The Journal of biological chemistry · 2012Article
- Response of fatty acid synthesis genes to the binding of human salivary amylase by Streptococcus gordonii.Applied and environmental microbiology · 2012Article
- Streptococcus pyogenes antigen I/II-family polypeptide AspA shows differential ligand-binding properties and mediates biofilm formation.Molecular microbiology · 2011Article
- Subgingival biofilm formation.Periodontology 2000 · 2010Review
- Development and optimization of a fluorescent differential display PCR system for analyzing the stress response in Lactobacillus sakei strains.Nutrients · 2009Article
- Salivary Genomics, Transcriptomics and Proteomics: The Emerging Concept of the Oral Ecosystem and their Use in the Early Diagnosis of Cancer and other Diseases.Current genomics · 2008Article
- Identification of genes regulated by changing salinity in the deep-sea bacterium Shewanella sp. WP3 using RNA arbitrarily primed PCR.Extremophiles : life under extreme conditions · 2006Article
- Inactivation of Streptococcus gordonii SspAB alters expression of multiple adhesin genes.Infection and immunity · 2005Article
- Identification of differential gene expression in bacteria associated with coral black band disease by using RNA-arbitrarily primed PCR.Applied and environmental microbiology · 2004Article
- Autoinducer 2 production by Streptococcus gordonii DL1 and the biofilm phenotype of a luxS mutant are influenced by nutritional conditions.Journal of bacteriology · 2003Article
- Communication among oral bacteria.Microbiology and molecular biology reviews : MMBR · 2002Review
- Identification of independent Streptococcus gordonii SspA and SspB functions in coaggregation with Actinomyces naeslundii.Infection and immunity · 2001Article
- Mutualism versus independence: strategies of mixed-species oral biofilms in vitro using saliva as the sole nutrient source.Infection and immunity · 2001Article
- Identification of stress-responsive genes in Streptococcus mutans by differential display reverse transcription-PCR.Infection and immunity · 2001Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Attachment of Streptococcus gordonii to the acquired pellicle of the tooth surface involves specific interactions between bacterial adhesins and adsorbed salivary components. To study saliva-regulated gene expression in S. gordonii, we used random arbitrarily primed PCR (RAP-PCR). Bacteria were incubated in either brain heart infusion medium or saliva. Total RNA from both conditions was purified and RAP fingerprinted and then PCR amplified with an arbitrary primer. The differentially displayed DNA fragments were cloned, sequenced, and analyzed using the BLAST search network service. Three DNA products were up-regulated. One was identified as that of the sspA and -B genes, which encode the salivary agglutinin glycoprotein-binding proteins SspA and SspB of S. gordonii; another had 79% identity with the Lactococcus lactis clpE gene, encoding a member of the Clp protease family; and the third product showed no significant homology to known genes. Five down-regulated genes were identified which encode proteins involved in bacterial metabolism. We have shown, for the first time, direct induction of sspA and -B in S. gordonii by human saliva.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.