ArticleInfection and immunity2000
Variability of outer membrane protein P1 and its evaluation as a vaccine candidate against experimental otitis media due to nontypeable Haemophilus influenzae: an unambiguous, multifaceted approach.
Article in Infection and immunity, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 45 citations in OpenAlex.
- Virulence factors ofFrontiers in microbiology · 2026Article
- Characterization of proteins present in the biofilm matrix and outer membrane vesicles ofmBio · 2025Article
- Antigen-specific memory Th17 cells promote cross-protection against nontypeable Haemophilus influenzae after mild influenza A virus infection.Mucosal immunology · 2023Article
- Immunogenicity of Nontypeable Haemophilus influenzae Outer Membrane Vesicles and Protective Ability in the Chinchilla Model of Otitis Media.Clinical and vaccine immunology : CVI · 2017Article
- Insights on persistent airway infection by non-typeable Haemophilus influenzae in chronic obstructive pulmonary disease.Pathogens and disease · 2017Review
- Nontypeable Haemophilus influenzae induces sustained lung oxidative stress and protease expression.PloS one · 2015Article
- Genome sequencing of disease and carriage isolates of nontypeable Haemophilus influenzae identifies discrete population structure.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- The role of the RNA chaperone Hfq in Haemophilus influenzae pathogenesis.BMC microbiology · 2013Article
- Intranasal immunization with nontypeable Haemophilus influenzae outer membrane vesicles induces cross-protective immunity in mice.PloS one · 2012Article
- Outer membrane protein A of bovine and ovine isolates of Mannheimia haemolytica is surface exposed and contains host species-specific epitopes.Infection and immunity · 2011Article
- Article
- Molecular genetic basis of ribotyping.Clinical microbiology reviews · 2008Article
- Analysis of genetic relatedness of Haemophilus influenzae isolates by multilocus sequence typing.Journal of bacteriology · 2008Article
- Glutathione-dependent alcohol dehydrogenase AdhC is required for defense against nitrosative stress in Haemophilus influenzae.Infection and immunity · 2007Article
- Multiple consecutive lavage samplings reveal greater burden of disease and provide direct access to the nontypeable Haemophilus influenzae biofilm in experimental otitis media.Infection and immunity · 2007Article
- Positively selected codons in immune-exposed loops of the vaccine candidate OMP-P1 of Haemophilus influenzae.Journal of molecular evolution · 2007Article
- Haemophilus influenzae phasevarions have evolved from type III DNA restriction systems into epigenetic regulators of gene expression.Nucleic acids research · 2007Article
- Host-derived sialic acid is incorporated into Haemophilus influenzae lipopolysaccharide and is a major virulence factor in experimental otitis media.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- High rates of recombination in otitis media isolates of non-typeable Haemophilus influenzae.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2003Article
- Postgenomic analysis of four novel antigens of group a streptococcus: growth phase-dependent gene transcription and human serologic response.Journal of bacteriology · 2002Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Candidate vaccine antigens for preventing otitis media caused by nontypeable Haemophilus influenzae (NTHI) should possess one or more conserved epitopes. We sought to evaluate the candidacy of P1, a surface-expressed outer membrane protein knowing that this antigen is subject to diversifying selection. Therefore, we selected NTHI strains from among >500 phylogenically variant isolates representative of the diversity found in natural populations of H. influenzae. Twenty-three variants of P1 (</=95% similarity) were identified among 42 strains. When chinchillas were immunized with recombinant P1 (rP1) obtained from one of these isolates (BCH-3), all animals developed antibodies specific for rP1. Immunized animals were protected against disease when challenged with BCH-3, but not with an ompP1 mutant of BCH-3 or a strain (BCH-2) possessing a heterologous P1 (91% identity). We conclude that (i) while P1 induces protection against NTHI-mediated otitis media, development of a polyvalent vaccine reflecting the variability of P1 would be necessary to construct an efficacious vaccine and (ii) use of a phylogenically characterized collection of representative isolates in concert with gene sequencing, cloning, gene inactivation, and animal testing offers an efficient, rational, and rigorous strategy for evaluating the potential problems associated with variability of vaccine targets and specificity of related immune responses.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.