ArticleGenome research2000
Molecular cloning, genomic organization, developmental regulation, and a knock-out mutant of a novel leu-rich repeats-containing G protein-coupled receptor (DLGR-2) from Drosophila melanogaster.
Article in Genome research, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 71 citations in OpenAlex.
- Evolutionary conserved peptide and glycoprotein hormone-like neuroendocrine systems in C. elegans.Molecular and cellular endocrinology · 2024Article
- Review: The evolution of peptidergic signaling in Cnidaria and Placozoa, including a comparison with Bilateria.Frontiers in endocrinology · 2022Review
- Signaling through the G-protein-coupled receptor Rickets is important for polarity, detachment, and migration of the border cells in Drosophila.Developmental biology · 2016Article
- RNAi-Mediated Functional Analysis of Bursicon Genes Related to Adult Cuticle Formation and Tanning in the Honeybee, Apis mellifera.PloS one · 2016Article
- Local control of intestinal stem cell homeostasis by enteroendocrine cells in the adult Drosophila midgut.Current biology : CB · 2014Article
- The heterodimeric glycoprotein hormone, GPA2/GPB5, regulates ion transport across the hindgut of the adult mosquito, Aedes aegypti.PloS one · 2014Article
- Membrane tethered bursicon constructs as heterodimeric modulators of the Drosophila G protein-coupled receptor rickets.Molecular pharmacology · 2013Article
- Amino acid sequence coevolution in the insect bursicon ligand-receptor system.Molecular phylogenetics and evolution · 2012Article
- A novel approach for directing transgene expression in Drosophila: T2A-Gal4 in-frame fusion.Genetics · 2012Article
- More than two decades of research on insect neuropeptide GPCRs: an overview.Frontiers in endocrinology · 2012Article
- Functional characterization of bursicon receptor and genome-wide analysis for identification of genes affected by bursicon receptor RNAi.Developmental biology · 2010Article
- Bursicon, the tanning hormone of insects: recent advances following the discovery of its molecular identity.Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology · 2008Review
- Conservation of the heterodimeric glycoprotein hormone subunit family proteins and the LGR signaling system from nematodes to humans.Endocrine · 2005Article
- Bursicon, the insect cuticle-hardening hormone, is a heterodimeric cystine knot protein that activates G protein-coupled receptor LGR2.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Neonatal lethality of LGR5 null mice is associated with ankyloglossia and gastrointestinal distension.Molecular and cellular biology · 2004Article
- Neuropeptides and neuropeptide receptors in the Drosophila melanogaster genome.Genome research · 2001Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
After screening the Berkeley Drosophila Genome Project database with sequences from a recently characterized Leu-rich repeats-containing G protein-coupled receptor (LGR) from Drosophila (DLGR-1), we identified a second gene for a different LGR (DLGR-2) and cloned its cDNA. DLGR-2 is 1360 amino acid residues long and shows a striking structural homology with members of the glycoprotein hormone [thyroid-stimulating hormone (TSH); follicle-stimulating hormone (FSH); luteinizing hormone/choriogonadotropin (LH/CG)] receptor family from mammals and with two additional, recently identified mammalian orphan LGRs (LGR-4 and LGR-5). This homology includes the seven transmembrane region (e.g., 49% amino acid identity with the human TSH receptor) and the very large extracellular amino terminus. This amino terminus contains 18 Leu-rich repeats-in contrast with the 3 mammalian glycoprotein hormone receptors and DLGR-1 that contain 9 Leu-rich repeats, but resembling the mammalian LGR-4 and LGR-5 that each have 17 Leu-rich repeats in their amino termini. The DLGR-2 gene is >18.6 kb pairs long and contains 15 exons and 14 introns. Four intron positions coincide with the intron positions of the three mammalian glycoprotein hormone receptors and have the same intron phasing, showing that DLGR-2 is evolutionarily related to these mammalian receptors. The DLGR-2 gene is located at position 34E-F on the left arm of the second chromosome and is expressed in embryos and pupae but not in larvae and adult flies. Homozygous knock-out mutants, where the DLGR-2 gene is interrupted by a P element insertion, die around the time of hatching. This finding, together with the expression data, strongly suggests that DLGR-2 is exclusively involved in development.
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