ArticleThe EMBO journal2000
Transcriptional termination and coupled polyadenylation in vitro.
Article in The EMBO journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed.
- Article
- PUFFFIN: an ultra-bright, customisable, single-plasmid system for labelling cell neighbourhoods.The EMBO journal · 2024Article
- Article
- Modulation of mRNA 3'-End Processing and Transcription Termination in Virus-Infected Cells.Frontiers in immunology · 2022Review
- Nrd1p identifies aberrant and natural exosomal target messages during the nuclear mRNA surveillance in Saccharomyces cerevisiae.Nucleic acids research · 2021Article
- The Myc-associated zinc finger protein (MAZ) works together with CTCF to control cohesin positioning and genome organization.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Emerging Roles of RNA 3'-end Cleavage and Polyadenylation in Pathogenesis, Diagnosis and Therapy of Human Disorders.Biomolecules · 2020Review
- mTERF8, a Member of the Mitochondrial Transcription Termination Factor Family, Is Involved in the Transcription Termination of Chloroplast GenePlant physiology · 2020Article
- Deficiency in the nuclear long noncoding RNAThe EMBO journal · 2018Article
- KDM5 lysine demethylases are involved in maintenance of 3'UTR length.Science advances · 2016Article
- Localization of RNAPII and 3' end formation factor CstF subunits on C. elegans genes and operons.Transcription · 2016Article
- mRNA quality control pathways in Saccharomyces cerevisiae.Journal of biosciences · 2013Review
- Viral and Cellular Components of AAV2 Replication Compartments.The open virology journal · 2013Article
- RNA processing and export.Cold Spring Harbor perspectives in biology · 2010Review
- Poly(A) signal-dependent degradation of unprocessed nascent transcripts accompanies poly(A) signal-dependent transcriptional pausing in vitro.RNA (New York, N.Y.) · 2010Article
- Transcription termination by nuclear RNA polymerases.Genes & development · 2009Article
- An evolutionary perspective of animal microRNAs and their targets.Journal of biomedicine & biotechnology · 2009Review
- G-quadruplexes: the beginning and end of UTRs.Nucleic acids research · 2008Article
- Glucocorticoid regulation of human pulmonary surfactant protein-B mRNA stability involves the 3'-untranslated region.American journal of respiratory cell and molecular biology · 2008Article
- The multifunctional protein p54nrb/PSF recruits the exonuclease XRN2 to facilitate pre-mRNA 3' processing and transcription termination.Genes & development · 2007Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using a coupled, in vitro transcription and polyadenylation system we have investigated the molecular mechanism of transcriptional termination by RNA polymerase II (PolII). We showed previously that specific G-rich sequences pause transcription and then activate polyadenylation. We show that physiological pause sites activate polyadenylation in our in vitro system. We also investigate the mechanism of PolII transcriptional termination, and show that these transcripts are either directly released from the transcription complex or are 3' end processed while still attached to the complex. We also show that 3' product (generated by cleavage/polyadenylation) remains associated with the transcription complex, but is rapidly degraded on it.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.