ArticleJournal of biomolecular screening2000
Novel fluorescence sensing methods for high throughput screening.
Article in Journal of biomolecular screening, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
5 citing papers in PubMed.
- Quantitative Particle Uptake by Cells as Analyzed by Different Methods.Angewandte Chemie (International ed. in English) · 2020Review
- Epigenetic assays for chemical biology and drug discovery.Clinical epigenetics · 2017Review
- Detection of hyaluronidase activity using fluorescein labeled hyaluronic acid and Fluorescence Correlation Spectroscopy.Journal of photochemistry and photobiology. B, Biology · 2012Article
- Enhanced fluorescence anisotropy assay for human cardiac troponin I and T detection.Journal of fluorescence · 2011Article
- Magnetically remote-controlled optical sensor spheres for monitoring oxygen or pH.Analytical chemistry · 2010Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
We describe two new methods of fluorescence sensing for use in high throughput screening (HTS). Modulation sensing transforms analyte-dependent intensity changes into a change in the low-frequency modulation signal. Polarization sensing transforms an intensity change into a change in polarization. Both methods are internally calibrated by using a reference film immediately adjacent to the sample, which can be readily located on the HTS plate or on a nearby optical component and provides an intensity or polarization reference. Modulation sensing and polarization sensing were both shown useful for measurements of fluorophore concentrations, pH, or calcium concentrations in the wells of HTS plates. Sensing with a reference film provides the opportunity to internally reference HTS measurements without the need for additions to the sample. This approach can provide standardization for assays performed at different times.
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Registered trials
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