ArticleJournal of bacteriology2000
Regulation of acetyl coenzyme A synthetase in Escherichia coli.
Article in Journal of bacteriology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 101 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
101 citing papers in PubMed, 226 citations in OpenAlex.
- Oxygen-dependent partitioning of acetate assimilation via Acs and AckA-Pta pathways inJournal of bacteriology · 2026Article
- Regulation of Escherichia coli fermentation processes: from molecular mechanisms to smart industrial practice.Archives of microbiology · 2026Review
- Breaking the membrane heredity paradox through de novo protocell formation.Nature communications · 2026Article
- Metabolic Engineering ofACS synthetic biology · 2025Article
- Carbon fluxes rewiring in engineered E. coli via reverse tricarboxylic acid cycle pathway under chemolithotrophic condition.Journal of biological engineering · 2025Article
- Acetyl-CoA synthetase activity is enzymatically regulated by lysine acetylation using acetyl-CoA or acetyl-phosphate as donor molecule.Nature communications · 2024Article
- Escherichia coli adaptation under prolonged resource exhaustion is characterized by extreme parallelism and frequent historical contingency.PLoS genetics · 2024Article
- Bacterial acetate metabolism and its influence on human epithelia.Emerging topics in life sciences · 2024Review
- Pairing metagenomics and metaproteomics to characterize ecological niches and metabolic essentiality of gut microbiomes.ISME communications · 2024Article
- Article
- Migration Rates on Swim Plates Vary between Escherichia coli Soil Isolates: Differences Are Associated with Variants in Metabolic Genes.Applied and environmental microbiology · 2023Article
- Adaptive laboratory evolution ofFrontiers in microbiology · 2023Article
- Negative regulation of the acsA1 gene encoding the major acetyl-CoA synthetase by cAMP receptor protein in Mycobacterium smegmatis.Journal of microbiology (Seoul, Korea) · 2022Article
- Coordinated expression of acetyl CoA synthetase and theMicrobiology (Reading, England) · 2022Article
- Article
- Mutants lacking global regulators, fis and arcA, in Escherichia coli enhanced growth fitness under acetate metabolism by pathway reprogramming.Applied microbiology and biotechnology · 2022Article
- Synergetic Fermentation of Glucose and Glycerol for High-Yield N-Acetylglucosamine Production inInternational journal of molecular sciences · 2022Article
- Response to substrate limitation by a marine sulfate-reducing bacterium.The ISME journal · 2022Article
- A lysate proteome engineering strategy for enhancing cell-free metabolite production.Metabolic engineering communications · 2021Article
- Unravelling the thioesterases responsible for propionate formation in engineered Pseudomonas putida KT2440.Microbial biotechnology · 2021Article
41 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cells of Escherichia coli growing on sugars that result in catabolite repression or amino acids that feed into glycolysis undergo a metabolic switch associated with the production and utilization of acetate. As they divide exponentially, these cells excrete acetate via the phosphotransacetylase-acetate kinase pathway. As they begin the transition to stationary phase, they instead resorb acetate, activate it to acetyl coenzyme A (acetyl-CoA) by means of the enzyme acetyl-CoA synthetase (Acs) and utilize it to generate energy and biosynthetic components via the tricarboxylic acid cycle and the glyoxylate shunt, respectively. Here, we present evidence that this switch occurs primarily through the induction of acs and that the timing and magnitude of this induction depend, in part, on the direct action of the carbon regulator cyclic AMP receptor protein (CRP) and the oxygen regulator FNR. It also depends, probably indirectly, upon the glyoxylate shunt repressor IclR, its activator FadR, and many enzymes involved in acetate metabolism. On the basis of these results, we propose that cells induce acs, and thus their ability to assimilate acetate, in response to rising cyclic AMP levels, falling oxygen partial pressure, and the flux of carbon through acetate-associated pathways.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.