ArticleThe Journal of cell biology2000
Glutamate slows axonal transport of neurofilaments in transfected neurons.
Article in The Journal of cell biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed, 168 citations in OpenAlex.
- Article
- Phosphorylated CRMP1, axon guidance protein, is a component of spheroids and is involved in axonal pathology in amyotrophic lateral sclerosis.Frontiers in neurology · 2022Article
- Retinal Spheroids and Axon Pathology Identified in Amyotrophic Lateral Sclerosis.Investigative ophthalmology & visual science · 2020Article
- Neurofilaments: neurobiological foundations for biomarker applications.Brain : a journal of neurology · 2020Review
- MicroRNA profiles of MS gray matter lesions identify modulators of the synaptic protein synaptotagmin-7.Brain pathology (Zurich, Switzerland) · 2020Article
- Anterograde Axonal Transport in Neuronal Homeostasis and Disease.Frontiers in molecular neuroscience · 2020Review
- Local Acceleration of Neurofilament Transport at Nodes of Ranvier.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- Upregulation of Vesicular Glutamate Transporter 2 and STAT3 Activation in the Spinal Cord of Mice Receiving 3,3'-Iminodipropionitrile.Neurotoxicity research · 2018Article
- Cytoskeleton as a Target of Quinolinic Acid Neurotoxicity: Insight from Animal Models.Molecular neurobiology · 2018Review
- Evaluation of Holistic Treatment for ALS Reveals Possible Mechanism and Therapeutic Potential.International journal of pharmacy and pharmaceutical research · 2017Article
- Neurobiology of axonal transport defects in motor neuron diseases: Opportunities for translational research?Neurobiology of disease · 2017Review
- Axonal transport deficits in multiple sclerosis: spiraling into the abyss.Acta neuropathologica · 2017Review
- Diagnostic and Prognostic Biomarkers in Amyotrophic Lateral Sclerosis: Neurofilament Light Chain Levels in Definite Subtypes of Disease.JAMA neurology · 2017Article
- Protein Homeostasis in Amyotrophic Lateral Sclerosis: Therapeutic Opportunities?Frontiers in molecular neuroscience · 2017Review
- Specialized roles of neurofilament proteins in synapses: Relevance to neuropsychiatric disorders.Brain research bulletin · 2016Review
- Protective and Toxic Neuroinflammation in Amyotrophic Lateral Sclerosis.Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics · 2015Review
- MFN2 couples glutamate excitotoxicity and mitochondrial dysfunction in motor neurons.The Journal of biological chemistry · 2015Article
- Miro1 deficiency in amyotrophic lateral sclerosis.Frontiers in aging neuroscience · 2015Article
- Divergent and convergent roles for kinases and phosphatases in neurofilament dynamics.Journal of cell science · 2014Article
- ER-mitochondria associations are regulated by the VAPB-PTPIP51 interaction and are disrupted by ALS/FTD-associated TDP-43.Nature communications · 2014Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Neurofilaments are transported through axons by slow axonal transport. Abnormal accumulations of neurofilaments are seen in several neurodegenerative diseases, and this suggests that neurofilament transport is defective. Excitotoxic mechanisms involving glutamate are believed to be part of the pathogenic process in some neurodegenerative diseases, but there is currently little evidence to link glutamate with neurofilament transport. We have used a novel technique involving transfection of the green fluorescent protein-tagged neurofilament middle chain to measure neurofilament transport in cultured neurons. Treatment of the cells with glutamate induces a slowing of neurofilament transport. Phosphorylation of the side-arm domains of neurofilaments has been associated with a slowing of neurofilament transport, and we show that glutamate causes increased phosphorylation of these domains in cell bodies. We also show that glutamate activates members of the mitogen-activated protein kinase family, and that these kinases will phosphorylate neurofilament side-arm domains. These results provide a molecular framework to link glutamate excitotoxicity with neurofilament accumulation seen in some neurodegenerative diseases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.