ArticleThe Journal of cell biology2000
The regulatory complex of Drosophila melanogaster 26S proteasomes. Subunit composition and localization of a deubiquitylating enzyme.
Article in The Journal of cell biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
What it found
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Who cites it
51 citing papers in PubMed, 147 citations in OpenAlex.
- Mechanisms and Design Principles of Proteolysis-Targeting Chimeras and Their Emerging Applications.ACS pharmacology & translational science · 2026Review
- The Structural Role of RPN10 in the 26S Proteasome and an RPN2-Binding Residue on RPN13 Are Functionally Important in Arabidopsis.International journal of molecular sciences · 2024Article
- PA200-Mediated Proteasomal Protein Degradation and Regulation of Cellular Senescence.International journal of molecular sciences · 2024Review
- A proteasomal β5 subunit of Haemonchus contortus with a role in the growth, development and life span.Parasites & vectors · 2023Article
- Plant deubiquitinases: from structure and activity to biological functions.Plant cell reports · 2023Review
- DNA origami signposts for identifying proteins on cell membranes by electron cryotomography.Cell · 2021Article
- Molecular Regulation of the Polycomb Repressive-Deubiquitinase.International journal of molecular sciences · 2020Review
- Development and application of ubiquitin-based chemical probes.Chemical science · 2020Review
- Role of deubiquitinases in DNA damage response.DNA repair · 2019Review
- Structure and Function of the 26S Proteasome.Annual review of biochemistry · 2018Review
- Unusual Antioxidant Properties of 26S Proteasome Isolated from Cold-Adapted Organisms.International journal of molecular sciences · 2017Article
- Genome sequence of Phormia regina Meigen (Diptera: Calliphoridae): implications for medical, veterinary and forensic research.BMC genomics · 2016Article
- Ubiquitin carboxyl-terminal hydrolase-L5 promotes TGFβ-1 signaling by de-ubiquitinating and stabilizing Smad2/Smad3 in pulmonary fibrosis.Scientific reports · 2016Article
- The initiator caspase Dronc is subject of enhanced autophagy upon proteasome impairment in Drosophila.Cell death and differentiation · 2016Article
- Uncommon functional properties of the first piscine 26S proteasome from the Antarctic notothenioid Trematomus bernacchii.Bioscience reports · 2016Article
- Structural basis for the activation and inhibition of the UCH37 deubiquitylase.Molecular cell · 2015Article
- Genome-wide screen for modifiers of Na (+) /K (+) ATPase alleles identifies critical genetic loci.Molecular brain · 2014Article
- Functions of the proteasome on chromatin.Biomolecules · 2014Review
- Emerging mechanistic insights into AAA complexes regulating proteasomal degradation.Biomolecules · 2014Review
- Effects of an anticarcinogenic Bowman-Birk protease inhibitor on purified 20S proteasome and MCF-7 breast cancer cells.PloS one · 2014Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Drosophila melanogaster embryos are a source for homogeneous and stable 26S proteasomes suitable for structural studies. For biochemical characterization, purified 26S proteasomes were resolved by two-dimensional (2D) gel electrophoresis and subunits composing the regulatory complex (RC) were identified by amino acid sequencing and immunoblotting, before corresponding cDNAs were sequenced. 17 subunits from Drosophila RCs were found to have homologues in the yeast and human RCs. An additional subunit, p37A, not yet described in RCs of other organisms, is a member of the ubiquitin COOH-terminal hydrolase family (UCH). Analysis of EM images of 26S proteasomes-UCH-inhibitor complexes allowed for the first time to localize one of the RC's specific functions, deubiquitylating activity. The masses of 26S proteasomes with either one or two attached RCs were determined by scanning transmission EM (STEM), yielding a mass of 894 kD for a single RC. This value is in good agreement with the summed masses of the 18 identified RC subunits (932 kD), indicating that the number of subunits is complete.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.