ArticleThe Journal of cell biology2000
Molecular basis for Rab prenylation.
Article in The Journal of cell biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 54 citations in OpenAlex.
- A Rab escort protein regulates the MAPK pathway that controls filamentous growth in yeast.Scientific reports · 2020Article
- GPCRs, G Proteins, and Their Impact on β-cell Function.Comprehensive Physiology · 2020Review
- Cell cycle-dependent phosphorylation of Sec4p controls membrane deposition during cytokinesis.The Journal of cell biology · 2016Article
- Choroideremia in a woman with ectodermal dysplasia and complex translocations involving chromosomes X, 1, and 3.Ophthalmic genetics · 2010Article
- Article
- Multiple domain insertions and losses in the evolution of the Rab prenylation complex.BMC evolutionary biology · 2007Article
- The Hsp90 chaperone complex regulates GDI-dependent Rab recycling.Molecular biology of the cell · 2006Article
- Structural basis for recruitment of RILP by small GTPase Rab7.The EMBO journal · 2005Article
- Mutation of the zebrafish choroideremia gene encoding Rab escort protein 1 devastates hair cells.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Molecular evolution of the Rab-escort-protein/guanine-nucleotide-dissociation-inhibitor superfamily.Molecular biology of the cell · 2003Article
- Arachidonate release and prostaglandin production by group IVC phospholipase A2 (cytosolic phospholipase A2gamma).The Biochemical journal · 2003Article
- Interactions of rotavirus VP4 spike protein with the endosomal protein Rab5 and the prenylated Rab acceptor PRA1.Journal of virology · 2003Article
- Rab2 GTPase regulates vesicle trafficking between the endoplasmic reticulum and the Golgi bodies and is important to pollen tube growth.The Plant cell · 2002Article
- A protein interaction map for cell polarity development.The Journal of cell biology · 2001Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Rab escort proteins (REP) 1 and 2 are closely related mammalian proteins required for prenylation of newly synthesized Rab GTPases by the cytosolic heterodimeric Rab geranylgeranyl transferase II complex (RabGG transferase). REP1 in mammalian cells is the product of the choroideremia gene (CHM). CHM/REP1 deficiency in inherited disease leads to degeneration of retinal pigmented epithelium and loss of vision. We now show that amino acid residues required for Rab recognition are critical for function of the yeast REP homologue Mrs6p, an essential protein that shows 50% homology to mammalian REPs. Mutant Mrs6p unable to bind Rabs failed to complement growth of a mrs6Delta null strain and were found to be dominant inhibitors of growth in a wild-type MRS6 strain. Mutants were identified that did not affect Rab binding, yet prevented prenylation in vitro and failed to support growth of the mrs6Delta null strain. These results suggest that in the absence of Rab binding, REP interaction with RabGG transferase is maintained through Rab-independent binding sites, providing a molecular explanation for the kinetic properties of Rab prenylation in vitro. Analysis of the effects of thermoreversible temperature-sensitive (mrs6(ts)) mutants on vesicular traffic in vivo showed prenylation activity is only transiently required to maintain normal growth, a result promising for therapeutic approaches to disease.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.