ArticleThe Journal of cell biology2000
Role of the cytoplasmic segments of Sec61alpha in the ribosome-binding and translocation-promoting activities of the Sec61 complex.
Article in The Journal of cell biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 69 citations in OpenAlex.
- Transgene-induced cell death following dengue-2 virus infection in Aedes aegypti.Scientific reports · 2023Article
- Sec61 complex/translocon: The role of an atypical ER CaFrontiers in physiology · 2022Review
- The role of X-box binding protein 1 in the hepatic response to refeeding in mice.Journal of lipid research · 2019Article
- Conserved motifs on the cytoplasmic face of the protein translocation channel are critical for the transition between resting and active conformations.The Journal of biological chemistry · 2018Article
- The Ribosome-Sec61 Translocon Complex Forms a Cytosolically Restricted Environment for Early Polytopic Membrane Protein Folding.The Journal of biological chemistry · 2015Article
- Transcriptional regulation of secretory capacity by bZip transcription factors.Frontiers in biology · 2015Article
- Article
- Protein translocation across the rough endoplasmic reticulum.Cold Spring Harbor perspectives in biology · 2013Review
- Subnuclear development of the zebrafish habenular nuclei requires ER translocon function.Developmental biology · 2011Article
- Derlin-dependent retrograde transport from endosomes to the Golgi apparatus.Traffic (Copenhagen, Denmark) · 2011Article
- SecA, a remarkable nanomachine.Cellular and molecular life sciences : CMLS · 2011Review
- Dynamics of SecY translocons with translocation-defective mutations.Structure (London, England : 1993) · 2010Article
- Structure of monomeric yeast and mammalian Sec61 complexes interacting with the translating ribosome.Science (New York, N.Y.) · 2009Article
- Regulation of the protein-conducting channel by a bound ribosome.Structure (London, England : 1993) · 2009Article
- Translocation of proteins through the Sec61 and SecYEG channels.Current opinion in cell biology · 2009Review
- A trans-membrane segment inside the ribosome exit tunnel triggers RAMP4 recruitment to the Sec61p translocase.The Journal of cell biology · 2009Article
- Constitutive, translation-independent opening of the protein-conducting channel in the endoplasmic reticulum.Pflugers Archiv : European journal of physiology · 2009Article
- Single copies of Sec61 and TRAP associate with a nontranslating mammalian ribosome.Structure (London, England : 1993) · 2008Article
- Protein translocation across the eukaryotic endoplasmic reticulum and bacterial plasma membranes.Nature · 2007Review
- Ribosome binding to and dissociation from translocation sites of the endoplasmic reticulum membrane.Molecular biology of the cell · 2006Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The Sec61 complex performs a dual function in protein translocation across the RER, serving as both the high affinity ribosome receptor and the translocation channel. To define regions of the Sec61 complex that are involved in ribosome binding and translocation promotion, ribosome-stripped microsomes were subjected to limited digestions using proteases with different cleavage specificities. Protein immunoblot analysis using antibodies specific for the NH(2) and COOH terminus of Sec61alpha was used to map the location of proteolysis cleavage sites. We observed a striking correlation between the loss of binding activity for nontranslating ribosomes and the digestion of the COOH- terminal tail or cytoplasmic loop 8 of Sec61alpha. The proteolyzed microsomes were assayed for SRP-independent translocation activity to determine whether high affinity binding of the ribosome to the Sec61 complex is a prerequisite for nascent chain transport. Microsomes that do not bind nontranslating ribosomes at physiological ionic strength remain active in SRP-independent translocation, indicating that the ribosome binding and translocation promotion activities of the Sec61 complex do not strictly correlate. Translocation-promoting activity was most severely inhibited by cleavage of cytosolic loop 6, indicating that this segment is a critical determinant for this function of the Sec61 complex.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.