ArticleThe Journal of cell biology2000
Reduced mobility of the alternate splicing factor (ASF) through the nucleoplasm and steady state speckle compartments.
Article in The Journal of cell biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
65 citing papers in PubMed, 174 citations in OpenAlex.
- Non-random inter-speckle material transfer mediated by dynamic multiphase interchromatin connections.The EMBO journal · 2026Article
- Membrane-less compartments in the nucleus: Separated or connected phases?Current opinion in cell biology · 2023Review
- Nuclear speckles - a driving force in gene expression.Journal of cell science · 2022Review
- Article
- Visualizing carboxyl-terminal domain of RNA polymerase II recruitment by FET fusion protein condensates with DNA curtains.Biophysics reports · 2022Article
- Revealing RCOR2 as a regulatory component of nuclear speckles.Epigenetics & chromatin · 2021Article
- Tyramide signal amplification mass spectrometry (TSA-MS) ratio identifies nuclear speckle proteins.The Journal of cell biology · 2020Article
- Availability of splicing factors in the nucleoplasm can regulate the release of mRNA from the gene after transcription.PLoS genetics · 2019Article
- Nuclear speckle fusion via long-range directional motion regulates speckle morphology after transcriptional inhibition.Journal of cell science · 2019Article
- rbFOX1/MBNL1 competition for CCUG RNA repeats binding contributes to myotonic dystrophy type 1/type 2 differences.Nature communications · 2018Article
- Casein kinase 2-mediated phosphorylation of Brahma-related gene 1 controls myoblast proliferation and contributes to SWI/SNF complex composition.The Journal of biological chemistry · 2017Article
- Coordinated Dynamics of RNA Splicing Speckles in the Nucleus.Journal of cellular physiology · 2016Article
- The in vivo dynamics of TCERG1, a factor that couples transcriptional elongation with splicing.RNA (New York, N.Y.) · 2016Article
- Regulation of mRNA export by the PI3 kinase/AKT signal transduction pathway.Molecular biology of the cell · 2013Article
- Heterochromatin instability in cancer: from the Barr body to satellites and the nuclear periphery.Seminars in cancer biology · 2013Review
- Identification and characterisation of Simiate, a novel protein linked to the fragile X syndrome.PloS one · 2013Article
- Changes in intranuclear mobility of mature snRNPs provide a mechanism for splicing defects in spinal muscular atrophy.Journal of cell science · 2012Article
- Compartmentalization of the nucleus.Trends in cell biology · 2011Review
- A density-dependent switch drives stochastic clustering and polarization of signaling molecules.PLoS computational biology · 2011Article
- Proteins on the move: insights gained from fluorescent protein technologies.Nature reviews. Molecular cell biology · 2011Review
5 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Compartmentalization of the nucleus is now recognized as an important level of regulation influencing specific nuclear processes. The mechanism of factor organization and the movement of factors in nuclear space have not been fully determined. Splicing factors, for example, have been shown to move in a directed manner as large intact structures from sites of concentration to sites of active transcription, but splicing factors are also thought to exist in a freely diffusible state. In this study, we examined the movement of a splicing factor, ASF, green fluorescent fusion protein (ASF-GFP) using time-lapse microscopy and the technique fluorescence recovery after photobleaching (FRAP). We find that ASF-GFP moves at rates up to 100 times slower than free diffusion when it is associated with speckles and, surprisingly, also when it is dispersed in the nucleoplasm. The mobility of ASF is consistent with frequent but transient interactions with relatively immobile nuclear binding sites. This mobility is slightly increased in the presence of an RNA polymerase II transcription inhibitor and the ASF molecules further enrich in speckles. We propose that the nonrandom organization of splicing factors reflects spatial differences in the concentration of relatively immobile binding sites.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.