ArticleProtein science : a publication of the Protein Society2000
Unfolding and refolding of cytochrome c driven by the interaction with lipid micelles.
Article in Protein science : a publication of the Protein Society, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- In silico study of cytochrome-C binding to a cardiolipin-containing membrane.European biophysics journal : EBJ · 2026Article
- Oleoyl coenzyme A triggers peroxygenase activity in cytochrome c.Journal of inorganic biochemistry · 2026Article
- Stability of uniformly labeled (Scientific reports · 2021Article
- The Puzzling Problem of Cardiolipin Membrane-Cytochrome c Interactions: A Combined Infrared and Fluorescence Study.International journal of molecular sciences · 2021Article
- Structural re-arrangement and peroxidase activation of cytochrome c by anionic analogues of vitamin E, tocopherol succinate and tocopherol phosphate.The Journal of biological chemistry · 2014Article
- Membrane-induced changes in the holomyoglobin tertiary structure: interplay with function.European biophysics journal : EBJ · 2014Article
- Observation of persistent α-helical content and discrete types of backbone disorder during a molten globule to ordered peptide transition via deep-UV resonance Raman spectroscopy.Journal of Raman spectroscopy : JRS · 2013Article
- Versatility of non-native forms of human cytochrome c: pH and micellar concentration dependence.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2013Article
- Topography of tyrosine residues and their involvement in peroxidation of polyunsaturated cardiolipin in cytochrome c/cardiolipin peroxidase complexes.Biochimica et biophysica acta · 2011Article
- The non-native conformations of cytochrome c in sodium dodecyl sulfate and their modulation by ATP.European biophysics journal : EBJ · 2011Article
- Cytochrome c/cardiolipin relations in mitochondria: a kiss of death.Free radical biology & medicine · 2009Review
- Interplay between bax, reactive oxygen species production, and cardiolipin oxidation during apoptosis.Biochemical and biophysical research communications · 2008Article
- Selective sensing of metalloproteins from nonselective binding using a fluorogenic amphiphilic polymer.Journal of the American Chemical Society · 2006Article
- Peroxidase activity and structural transitions of cytochrome c bound to cardiolipin-containing membranes.Biochemistry · 2006Article
- Article
- Conformational equilibria and dynamics of cytochrome c induced by binding of sodium dodecyl sulfate monomers and micelles.European biophysics journal : EBJ · 2003Article
- Two-dimensional infrared correlation spectroscopy study of the aggregation of cytochrome c in the presence of dimyristoylphosphatidylglycerol.Biophysical journal · 2001Article
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2 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Binding of native cyt c to L-PG micelles leads to a partially unfolded conformation of cyt c. This micelle-bound state has no stable tertiary structure, but remains as alpha-helical as native cyt c in solution. In contrast, binding of the acid-unfolded cyt c to L-PG micelles induces folding of the polypeptide, resulting in a similar helical state to that originated from the binding of native cyt c to L-PG micelles. Far-ultraviolet (UV) circular dichroism (CD) spectra showed that this common micelle-associated helical state (HL) has a native-like alpha-helix content, but is highly expanded without a tightly packed hydrophobic core, as revealed by tryptophan fluorescence, near-UV, and Soret CD spectroscopy. The kinetics of the interaction of native and acid-unfolded cyt c was investigated by stopped-flow tryptophan fluorescence. Formation of H(L) from the native state requires the disruption of the tightly packed hydrophobic core in the native protein. This micelle-induced unfolding of cyt c occurs at a rate approximately 0.1 s(-1), which is remarkably faster in the lipid environment compared with the expected rate of unfolding in solution. Refolding of acid-unfolded cyt c with L-PG micelles involves an early highly helical collapsed state formed during the burst phase (<3 ms), and the observed main kinetic event reports on the opening of this early compact intermediate prior to insertion into the lipid micelle.
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