ArticleMolecular and cellular biology2000
Long-distance control of origin choice and replication timing in the human beta-globin locus are independent of the locus control region.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
47 citing papers in PubMed, 114 citations in OpenAlex.
- Rho enhancers play unexpectedly minor roles in Rhodopsin transcription and rod cell integrity.Scientific reports · 2023Article
- Replication Stress, Genomic Instability, and Replication Timing: A Complex Relationship.International journal of molecular sciences · 2021Review
- TheProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Control of DNA replication timing in the 3D genome.Nature reviews. Molecular cell biology · 2019Review
- Human NOC3 is essential for DNA replication licensing in human cells.Cell cycle (Georgetown, Tex.) · 2019Article
- Article
- Maintenance of Epigenetic Information.Cold Spring Harbor perspectives in biology · 2016Review
- Altered histone modifications in gliomas.Brain tumor research and treatment · 2014Review
- Alterations in replication timing of cancer-related genes in malignant human breast cancer cells.Journal of cellular biochemistry · 2013Article
- DNA replication timing, genome stability and cancer: late and/or delayed DNA replication timing is associated with increased genomic instability.Seminars in cancer biology · 2013Review
- Replication-timing boundaries facilitate cell-type and species-specific regulation of a rearranged human chromosome in mouse.Human molecular genetics · 2012Article
- Kaposi's Sarcoma-Associated Herpesvirus Genome Replication, Partitioning, and Maintenance in Latency.Frontiers in microbiology · 2012Article
- Interactions of MCP1 with components of the replication machinery in mammalian cells.International journal of biological sciences · 2011Article
- Chromatin landscape: methylation beyond transcription.Epigenetics · 2011Review
- Gene density profile reveals the marking of late replicated domains in the Drosophila melanogaster genome.Chromosoma · 2010Article
- A multiprotein complex necessary for both transcription and DNA replication at the β-globin locus.The EMBO journal · 2010Article
- Differences in the epigenetic and reprogramming properties of pluripotent and extra-embryonic stem cells implicate chromatin remodelling as an important early event in the developing mouse embryo.Epigenetics & chromatin · 2010Article
- Domain-wide regulation of DNA replication timing during mammalian development.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2010Article
- Chromatin state marks cell-type- and gender-specific replication of the Drosophila genome.Genes & development · 2009Article
- Replication timing as an epigenetic mark.Epigenetics · 2009Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
DNA replication in the human beta-globin locus is subject to long-distance regulation. In murine and human erythroid cells, the human locus replicates in early S phase from a bidirectional origin located near the beta-globin gene. This Hispanic thalassemia deletion removes regulatory sequences located over 52 kb from the origin, resulting in replication of the locus from a different origin, a shift in replication timing to late S phase, adoption of a closed chromatin conformation, and silencing of globin gene expression in murine erythroid cells. The sequences deleted include nuclease-hypersensitive sites 2 to 5 (5'HS2-5) of the locus control region (LCR) plus an additional 27-kb upstream region. We tested a targeted deletion of 5'HS2-5 in the normal chromosomal context of the human beta-globin locus to determine the role of these elements in replication origin choice and replication timing. We demonstrate that the 5'HS2-5-deleted locus initiates replication at the appropriate origin and with normal timing in murine erythroid cells, and therefore we conclude that 5'HS2-5 in the classically defined LCR do not control replication in the human beta-globin locus. Recent studies also show that targeted deletion of 5'HS2-5 results in a locus that lacks globin gene expression yet retains an open chromatin conformation. Thus, the replication timing of the locus is closely correlated with nuclease sensitivity but not globin gene expression.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.