ArticleMolecular and cellular biology2000
Shared roles of yeast glycogen synthase kinase 3 family members in nitrogen-responsive phosphorylation of meiotic regulator Ume6p.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 57 citations in OpenAlex.
- Multi-signal regulation of the GSK-3β homolog Rim11 controls meiosis entry in budding yeast.The EMBO journal · 2024Article
- Transcriptome Analysis Reveals Mycelial and Fruiting Responses to Lithium Chloride inJournal of fungi (Basel, Switzerland) · 2024Article
- Yeast Rim11 kinase responds to glutathione-induced stress by regulating the transcription of phospholipid biosynthetic genes.Molecular biology of the cell · 2024Article
- Ume6 Acts as a Stable Platform To Coordinate Repression and Activation of Early Meiosis-Specific Genes in Saccharomyces cerevisiae.Molecular and cellular biology · 2021Article
- Phosphorylation of the Gα protein Gpa2 promotes protein kinase A signaling in yeast.The Journal of biological chemistry · 2019Article
- Developmentally regulated internal transcription initiation during meiosis in budding yeast.PloS one · 2017Article
- Nitrogen Starvation-induced Phosphorylation of Ras1 Protein and Its Potential Role in Nutrient Signaling and Stress Response.The Journal of biological chemistry · 2016Article
- Fgk3 glycogen synthase kinase is important for development, pathogenesis, and stress responses in Fusarium graminearum.Scientific reports · 2015Article
- The Rim15-endosulfine-PP2ACdc55 signalling module regulates entry into gametogenesis and quiescence via distinct mechanisms in budding yeast.PLoS genetics · 2014Article
- Dynamic modeling of yeast meiotic initiation.BMC systems biology · 2013Article
- Ume6 transcription factor is part of a signaling cascade that regulates autophagy.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Ime1 and Ime2 are required for pseudohyphal growth of Saccharomyces cerevisiae on nonfermentable carbon sources.Molecular and cellular biology · 2010Article
- Ume6 is required for the MATa/MATalpha cellular identity and transcriptional silencing in Kluyveromyces lactis.Genetics · 2010Article
- Effects of age on meiosis in budding yeast.Developmental cell · 2009Article
- Meiosis-specific destruction of the Ume6p repressor by the Cdc20-directed APC/C.Molecular cell · 2007Article
- Interplay between chromatin and trans-acting factors on the IME2 promoter upon induction of the gene at the onset of meiosis.Molecular and cellular biology · 2007Article
- Rim15 and the crossroads of nutrient signalling pathways in Saccharomyces cerevisiae.Cell division · 2006Article
- Glucose inhibits meiotic DNA replication through SCFGrr1p-dependent destruction of Ime2p kinase.Molecular and cellular biology · 2005Article
- The in vivo activity of Ime1, the key transcriptional activator of meiosis-specific genes in Saccharomyces cerevisiae, is inhibited by the cyclic AMP/protein kinase A signal pathway through the glycogen synthase kinase 3-beta homolog Rim11.Molecular and cellular biology · 2004Article
- Glucose and nitrogen regulate the switch from histone deacetylation to acetylation for expression of early meiosis-specific genes in budding yeast.Molecular and cellular biology · 2004Article
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Authors and funding
2 authors at 1 institution in 2 countries.
Funding
Abstract
Nitrogen limitation activates meiosis and meiotic gene expression in yeast, but nitrogen-responsive signal transduction mechanisms that govern meiotic gene expression are poorly understood. We show here that Ume6p, a subunit of the Ume6p-Ime1p meiotic transcriptional activator, undergoes increased phosphorylation in vivo in response to nitrogen limitation. Phosphorylation depends on an N-terminal glycogen synthase kinase 3 (GSK3) target site in which substitutions cause reduced Ume6p-Ime1p interaction and meiotic gene expression, thus arguing that phosphorylation promotes functional Ume6p-Ime1p interaction. Phosphorylation of this site depends on two GSK3 homologs, Rim11p and Mck1p. Prior studies indicate that Rim11p phosphorylates both Ume6p and Ime1p in vitro and is required for Ume6p-Ime1p interaction, but no evidence has linked Mck1p function to Ume6p activity. Here we find that Mck1p-Ume6p interaction is detectable by two-hybrid assays and that meiosis in a partially defective rim11-K68R mutant is completely dependent on Mck1p. These findings argue that nitrogen limitation governs Rim11p/Mck1p-dependent phosphorylation of Ume6p, which in turn is required for Ume6p-Ime1p interaction and meiotic gene activation.
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