ArticlePlant molecular biology2000
Characterization of a small GTP-binding protein of the rab 5 family in Mesembryanthemum crystallinum with increased level of expression during early salt stress.
Article in Plant molecular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 80 citations in OpenAlex.
- Protein Prenylation in Plants: Mechanisms and Functional Implications.Plants (Basel, Switzerland) · 2025Review
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- Genome-Wide Analysis of theInternational journal of molecular sciences · 2022Article
- Transcriptome Reveals the Dynamic Response Mechanism of Pearl Millet Roots under Drought Stress.Genes · 2021Article
- Plant RABs: Role in Development and in Abiotic and Biotic Stress Responses.Current genomics · 2021Review
- Salt-induced expression of intracellular vesicle trafficking genes, CaRab-GTP, and their association with NaBMC plant biology · 2020Article
- TheGenes · 2019Article
- Intracellular Vesicle Trafficking Genes,Frontiers in genetics · 2019Article
- Article
- NbRABG3f, a member of Rab GTPase, is involved in Bamboo mosaic virus infection in Nicotiana benthamiana.Molecular plant pathology · 2016Article
- CELLULOSE SYNTHASE INTERACTIVE1 Is Required for Fast Recycling of Cellulose Synthase Complexes to the Plasma Membrane in Arabidopsis.The Plant cell · 2015Article
- Molecular Analysis and Localization of CaARA7 a Conventional RAB5 GTPase from Characean Algae.Traffic (Copenhagen, Denmark) · 2015Article
- Molecular characterization and identification of target protein of an important vesicle trafficking gene AlRab7 from a salt excreting halophyte Aeluropus lagopoides.DNA and cell biology · 2015Article
- Molecular and biochemical analysis of the first ARA6 homologue, a RAB5 GTPase, from green algae.Journal of experimental botany · 2013Article
- The small GTPase ROP6 interacts with NFR5 and is involved in nodule formation in Lotus japonicus.Plant physiology · 2012Article
- Conserved and plant-unique mechanisms regulating plant post-Golgi traffic.Frontiers in plant science · 2012Article
- Legume small GTPases and their role in the establishment of symbiotic associations with Rhizobium spp.Plant signaling & behavior · 2009Review
- Identification of an apoplastic protein involved in the initial phase of salt stress response in rice root by two-dimensional electrophoresis.Plant physiology · 2009Article
- Constitutive overexpression of a stress-inducible small GTP-binding protein PgRab7 from Pennisetum glaucum enhances abiotic stress tolerance in transgenic tobacco.Plant cell reports · 2008Article
- Comparative phylogenetic analysis of small GTP-binding genes of model legume plants and assessment of their roles in root nodules.Journal of experimental botany · 2008Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A cDNA encoding a member of the Ypt/Rab family of small GTP-binding proteins was cloned from the facultative CAM plant Mesembryanthemum crystallinum. Mcrab5b includes an open reading frame of 201 amino acids. The deduced amino acid sequence shows 91% similarity to LjRAB5b isolated from Lotus japonicus. The amino acid sequence of McRAB5b provides interesting features suggesting that McRAB5b and its homologue from Lotus japonicus represent a new subclass of Ypt/Rab proteins. The fact that proteins like McRAB5b and LjRAB5b were only found in plants and not in yeast or vertebrates suggests that they have plant-specific functions. The expression of Mcrab5b as investigated by northern blot hybridization and RT-PCR was stimulated under salt stress. After heterologous expression in Escherichia coli an antibody was raised against recombinant McRABSb protein. Western blot analysis revealed that McRAB5b was bound to membranes. It is present in a monomeric and a dimeric form in vitro and in vivo. In vitro only the monomeric protein exhibits a binding capacity for radiolabelled GTP, while the dimer is unable to do so, indicating that the activity may be regulated by monomer/dimer transition.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.