ArticleJournal of clinical pathology2000
Comparison of in house polymerase chain reaction with conventional techniques for the detection of Mycobacterium tuberculosis DNA in granulomatous lymphadenopathy.
Article in Journal of clinical pathology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 180 citations in OpenAlex.
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- Efficacy of cartridge-based nucleic acid amplification test (CBNAAT) in comparison with line probe assay and liquid culture in the evaluation of tubercular lymph node abscess.Journal of family medicine and primary care · 2024Article
- Cure Rates for Tuberculous Cervical Lymphadenopathy after 6-month or 9-month Anti-tuberculous Therapy.Acta medica Philippina · 2024Article
- Clinical characteristics and outcome of Tuberculosis lymphadenitis in a tertiary center from Saudi Arabia.Journal of clinical tuberculosis and other mycobacterial diseases · 2023Article
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- Comparative Diagnostic of Cervical Tuberculous Lymphadenitis: PCR is a Fast, Efficient, and Improved Diagnostic Approach.The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2023Article
- Primary hepatic tuberculosis presenting as fever of unknown origin diagnosed by Positron emission tomography/computed tomography scan (PET/CT).Journal of family medicine and primary care · 2022Article
- Misidentification of hepatic tuberculosis as cholangiocarcinoma: A case report.World journal of clinical cases · 2021Article
- A molecular strategy to optimize bovine tuberculosis post-mortem diagnosis and the exposure to Mycobacterium tuberculosis variant bovis.Molecular biology reports · 2020Article
- Tuberculosis as a differential diagnosis of salivary gland malignancy.BMJ case reports · 2020Article
- Determining the optimal puncture site of CT-guided transthoracic needle aspiration biopsy for the diagnosis of tuberculosis.Journal of thoracic disease · 2020Article
- Looking beyond appearances: when liver biopsy is the key for hepatic tuberculosis diagnosis.BMJ case reports · 2020Article
- Diagnostic accuracy of the Xpert MTB/Rif Ultra for tuberculosis adenitis.BMC infectious diseases · 2020Article
- Primary hepatic tuberculosis: A rare occurrence.Annals of hepato-biliary-pancreatic surgery · 2019Article
- Primary Tubercular Sialadenitis - A Diagnostic Dilemma.Iranian journal of otorhinolaryngology · 2019Article
- Pathogenic Leptospira spp. in bats: Molecular investigation in Southern Brazil.Comparative immunology, microbiology and infectious diseases · 2017Article
- Differentiation of Mycobacterium tuberculosis complex from non-tubercular mycobacteria by nested multiplex PCR targeting IS6110, MTP40 and 32kD alpha antigen encoding gene fragments.BMC infectious diseases · 2016Article
- Endoscopic ultrasound characteristics of tubercular lymphadenopathy in comparison to reactive lymph nodes.Indian journal of gastroenterology : official journal of the Indian Society of Gastroenterology · 2016Article
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
aimsTo evaluate the usefulness of the devR based polymerase chain reaction (PCR) in the detection of Mycobacterium tuberculosis in lymph node aspirates and tissues of lymphadenitis and to compare PCR with conventional diagnostic techniques. SUBJECTS AND
methodsCoded specimens of fine needle aspirates and biopsies from 22 patients with tuberculous lymphadenitis, 14 patients with non-tubercular lymphadenitis, and nine patients with granulomatous lymphadenitis were processed and subjected to analysis by PCR, smear microscopy, M tuberculosis culture, histology, and cytology.
resultsTuberculous lymphadenitis was correctly diagnosed by PCR in 18 patients, by culture in five patients, by histology in 13 patients, and by cytology in seven patients. PCR gave two false positive results in 14 patients with non-tubercular lymphadenitis. The sensitivity of the conventional techniques was significantly higher with biopsies (17 of 22 specimens; 77%) than with fine needle aspirates (nine of 22 specimens; 41%). However, the sensitivity of PCR was not significantly higher with biopsies (68%) in comparison with fine needle aspirates (55%). The sensitivity of either biopsy PCR or fine needle aspirate PCR was not significantly different from that of either histology combined with culture or cytology combined with culture. The overall combined specificity of PCR was 86%. Mycobacterium tuberculosis DNA was detected in six of nine patients with granulomatous lymphadenitis.
conclusionPCR is the most sensitive single technique available to date for the demonstration of M tuberculosis in specimens derived from patients with a clinical suspicion of tuberculous lymphadenitis. The value of PCR lies in its use as an adjunct test in the diagnosis of tuberculous lymphadenitis, particularly in those patients where conventional methods fail. Because fine needle aspiration is not an invasive procedure, it is the procedure of choice, and PCR should be performed initially on these samples. Excisional biopsy histology and PCR should be recommended only for patients in whom fine needle aspirate PCR is negative or when there is discrepancy with the clinical impression.
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