ArticlePlant physiology2000
Isolation and characterization of plant N-acetyl glucosaminyltransferase I (GntI) cDNA sequences. Functional analyses in the Arabidopsis cgl mutant and in antisense plants.
Article in Plant physiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 64 citations in OpenAlex.
- Recent Developments in Deciphering the Biological Role of Plant ComplexFrontiers in plant science · 2022Review
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- ComplexFrontiers in plant science · 2021Article
- Inactivation ofFrontiers in plant science · 2021Article
- Establishment of a tobacco BY2 cell line devoid of plant-specific xylose and fucose as a platform for the production of biotherapeutic proteins.Plant biotechnology journal · 2017Article
- Heterologous expression of the N-acetylglucosaminyltransferase I dictates a reinvestigation of the N-glycosylation pathway in Chlamydomonas reinhardtii.Scientific reports · 2017Article
- The production of human glucocerebrosidase in glyco-engineered Nicotiana benthamiana plants.Plant biotechnology journal · 2016Article
- Characterization of plants expressing the human β1,4-galactosyltrasferase gene.Plant physiology and biochemistry : PPB · 2015Article
- RISAP is a TGN-associated RAC5 effector regulating membrane traffic during polar cell growth in tobacco.The Plant cell · 2014Article
- OsMOGS is required for N-glycan formation and auxin-mediated root development in rice (Oryza sativa L.).The Plant journal : for cell and molecular biology · 2014Article
- Cell wall O-glycoproteins and N-glycoproteins: aspects of biosynthesis and function.Frontiers in plant science · 2014Review
- Characterization and downstream mannose phosphorylation of human recombinant α-L-iduronidase produced in Arabidopsis complex glycan-deficient (cgl) seeds.Plant biotechnology journal · 2013Article
- The Fusarium oxysporum gnt2, encoding a putative N-acetylglucosamine transferase, is involved in cell wall architecture and virulence.PloS one · 2013Article
- The secreted plant N-glycoproteome and associated secretory pathways.Frontiers in plant science · 2012Article
- Expression of functional recombinant human growth hormone in transgenic soybean seeds.Transgenic research · 2011Article
- Reduced immunogenicity of Arabidopsis hgl1 mutant N-glycans caused by altered accessibility of xylose and core fucose epitopes.The Journal of biological chemistry · 2011Article
- Article
- Differential effects of human and plant N-acetylglucosaminyltransferase I (GnTI) in plants.Transgenic research · 2010Article
- Isoenzyme replacement of glucose-6-phosphate dehydrogenase in the cytosol improves stress tolerance in plants.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Comparative analyses of Arabidopsis complex glycan1 mutants and genetic interaction with staurosporin and temperature sensitive3a.Plant physiology · 2008Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report on the isolation and characterization of full-length cDNA sequences coding for N-acetylglucosaminyltransferase I (GnTI) from potato (Solanum tuberosum L.), tobacco (Nicotiana tabacum L.), and Arabidopsis. The deduced polypeptide sequences show highest homology among the solanaceous species (93% identity between potato and tobacco compared with about 75% with Arabidopsis) but share only weak homology with human GnTI (35% identity). In contrast to the corresponding enzymes from animals, all plant GnTI sequences identified are characterized by a much shorter hydrophobic membrane anchor and contain one putative N-glycosylation site that is conserved in potato and tobacco, but differs in Arabidopsis. Southern-blot analyses revealed that GntI behaves as a single-copy gene. Northern-blot analyses showed that GntI-mRNA expression is largely constitutive. Arabidopsis cgl mutants deficient in GnTI activity also possess GntI mRNA, indicating that they result from point mutations. GntI-expression constructs were tested for the ability to relieve the GnTI block in protoplasts of the Arabidopsis cgl mutant and used to obtain transgenic potato and tobacco plants that display a substantial reduction of complex glycan patterns. The latter observation indicates that production of heterologous glycoproteins with little or no antigenic glycans can be achieved in whole plants, and not in just Arabidopsis, using antisense technology.
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