ArticlePlant physiology2000
Characterization of sulfate assimilation in marine algae focusing on the enzyme 5'-adenylylsulfate reductase.
Article in Plant physiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 60 citations in OpenAlex.
- Catalysts for sulfur: understanding the intricacies of enzymes orchestrating plant sulfur anabolism.Planta · 2024Review
- Effects of seawater sulfur starvation and enrichment on Gracilaria gracilis growth and biochemical composition.Scientific reports · 2022Article
- Review
- Sulfur metabolites in the pelagic ocean.Nature reviews. Microbiology · 2019Review
- Variability in sulfur isotope composition suggests unique dimethylsulfoniopropionate cycling and microalgae metabolism in Antarctic sea ice.Communications biology · 2018Article
- Transcriptome profiling of sulfate deprivation responses in two agarophytes Gracilaria changii and Gracilaria salicornia (Rhodophyta).Scientific reports · 2017Article
- Phylogeny of Algal Sequences Encoding Carbohydrate Sulfotransferases, Formylglycine-Dependent Sulfatases, and Putative Sulfatase Modifying Factors.Frontiers in plant science · 2015Article
- Article
- Amino Acid biosynthesis pathways in diatoms.Metabolites · 2013Article
- Plant sulfate assimilation genes: redundancy versus specialization.Plant cell reports · 2009Review
- Interaction domain on thioredoxin for Pseudomonas aeruginosa 5'-adenylylsulfate reductase.The Journal of biological chemistry · 2009Article
- Sulfate assimilation in eukaryotes: fusions, relocations and lateral transfers.BMC evolutionary biology · 2008Article
- Sulfur and primary production in aquatic environments: an ecological perspective.Photosynthesis research · 2005Review
- The role of 5'-adenylylsulfate reductase in controlling sulfate reduction in plants.Photosynthesis research · 2005Review
- Sulfur assimilation and the role of sulfur in plant metabolism: a survey.Photosynthesis research · 2004Article
- The sac mutants of Chlamydomonas reinhardtii reveal transcriptional and posttranscriptional control of cysteine biosynthesis.Plant physiology · 2002Article
- Sulfate metabolism.The arabidopsis book · 2002Article
- Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
5'-Adenylylsulfate (APS) reductase was characterized in diverse marine algae. A cDNA encoding APS reductase from Enteromorpha intestinalis (EAPR) was cloned by functional complementation of an Escherichia coli cysH mutant. The deduced amino acid sequence shows high homology with APS reductase (APR) from flowering plants. Based on the probable transit peptide cleavage site the mature protein is 45.7 kD. EAPR expressed as a His-tagged recombinant protein catalyzes reduced glutathione-dependent reduction of APS to sulfite, exhibiting a specific activity of approximately 40 micromol min(-1) mg protein(-1) and Michealis-Menten kinetic constants of approximately 1.4 mM for reduced glutathione and approximately 6.5 microM for APS. APR activity and expression were studied in relation to the production of 3-dimethylsulfoniopropionate (DMSP), a sulfonium compound produced by many marine algae. A diverse group of DMSP-producing species showed extremely high enzyme activity (up to 400 times that found in flowering plants). Antibodies raised against a conserved peptide of APR strongly cross-reacted with a protein of 45 kD in several chlorophytes but insignificantly with chromophytes. In the chlorophyte Tetraselmis sp., APR activity varies significantly during the culture cycle and does not follow the changes in cellular DMSP content. However, a positive correlation was found between cell-based APR activity and specific growth rate.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.