ArticlePlant physiology2000
Increasing tryptophan synthesis in a forage legume Astragalus sinicus by expressing the tobacco feedback-insensitive anthranilate synthase (ASA2) gene.
Article in Plant physiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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27 citing papers in PubMed, 77 citations in OpenAlex.
- Evolution of aromatic amino acid metabolism in plants: a key driving force behind plant chemical diversity in aromatic natural products.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Review
- Hydrotime Model Parameters Estimate Seed Vigor and Predict Seedling Emergence Performance ofPlants (Basel, Switzerland) · 2023Article
- Phytochrome interacting factor 3 regulates pollen mitotic division through auxin signalling and sugar metabolism pathways in tomato.The New phytologist · 2022Article
- Genetic engineering to improve essential and conditionally essential amino acids in maize: transporter engineering as a reference.Transgenic research · 2021Article
- Physico-chemical characterization and transcriptome analysis of 5-methyltryptophan resistant lines in rice.PloS one · 2019Article
- Fortifying Horticultural Crops with Essential Amino Acids: A Review.International journal of molecular sciences · 2017Review
- Improving Nutritional Quality of Plant Proteins Through Genetic Engineering.Current genomics · 2016Article
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- Transcriptional network analysis of the tryptophan-accumulating rice mutant during grain filling.Molecular genetics and genomics : MGG · 2012Article
- Increased glutamine in leaves of poplar transgenic with pine GS1a caused greater anthranilate synthetase α-subunit (ASA1) transcript and protein abundances: an auxin-related mechanism for enhanced growth in GS transgenics?Journal of experimental botany · 2011Article
- Tobacco plastid transformation using the feedback-insensitive anthranilate synthase [alpha]-subunit of tobacco (ASA2) as a new selectable marker.Journal of experimental botany · 2009Article
- Tissue culture specificity of the tobacco ASA2 promoter driving hpt as a selectable marker for soybean transformation selection.Plant cell reports · 2008Article
- Use of 4-methylindole or 7-methyl-DL-tryptophan in a transformant selection system based on the feedback-insensitive anthranilate synthase alpha-subunit of tobacco (ASA2).Plant cell reports · 2008Article
- Expression of a feedback insensitive anthranilate synthase gene from tobacco increases free tryptophan in soybean plants.Plant cell reports · 2007Article
- Plant native tryptophan synthase beta 1 gene is a non-antibiotic selection marker for plant transformation.Planta · 2007Article
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- Chorismate derived C6C1 compounds in plants.Planta · 2005Review
- Structure-based in vitro engineering of the anthranilate synthase, a metabolic key enzyme in the plant tryptophan pathway.Plant physiology · 2005Article
- Overexpression of the feedback-insensitive anthranilate synthase gene in tobacco causes tryptophan accumulation.Plant cell reports · 2005Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A cDNA clone that encodes a feedback-insensitive anthranilate synthase (AS), ASA2, isolated from a 5-methyl-tryptophan (Trp) (5MT)-resistant tobacco cell line under the control of the constitutive cauliflower mosaic virus 35S promoter, was introduced into the forage legume Astragalus sinicus by Agrobacterium rhizogenes with kanamycin selection. The 35S-ASA2 gene was expressed constitutively as demonstrated by northern-blot hybridization analyses and the presence of feedback-insensitive AS. Hairy root lines transformed with 35S-ASA2 grew in concentrations of up to 100 microM 5MT, whereas the controls were completely inhibited by 15 microM 5MT. Expression of the feedback-insensitive ASA2 resulted in a 1.3- to 5.5-fold increase in free Trp. Kinetic studies of the AS activity demonstrate the Trp feedback alterations and indicate that the ASA2 alpha-subunit can interact with the native A. sinicus beta-subunit to form an active enzyme. The ASA2 transcript and high free Trp were also detected in the leaves, stems, and roots of plants regenerated from the transformed hairy roots. Thus, we show for the first time that ASA2 can be used to transform plants of a different species to increase the levels of the essential amino acid Trp and impart 5MT resistance.
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