ArticlePlant physiology2000
Tissue-preferential expression of a rice alpha-tubulin gene, OsTubA1, mediated by the first intron.
Article in Plant physiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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52 citing papers in PubMed.
- Characterization ofSynthetic and systems biotechnology · 2027Article
- Engineering non-coding DNA Elements in Rice: an Elegant Approach To fine-tune Agronomical Advantageous Traits.Rice (New York, N.Y.) · 2025Review
- Genome-wide identification of the PI-PLC gene family inFrontiers in plant science · 2025Article
- Chalkiness and premature controlled by energy homeostasis in OsNAC02 Ko-mutant during vegetative endosperm development.BMC plant biology · 2024Article
- Integrated analysis strategy of genome-wide functional gene mining reveals DKK2 gene underlying meat quality in Shaziling synthesized pigs.BMC genomics · 2024Article
- Genome-Wide Identification and Expression Analysis ofPlants (Basel, Switzerland) · 2022Article
- Prospects of Non-Coding Elements in Genomic DNA Based Gene Therapy.Current gene therapy · 2022Review
- Proximity to the Promoter and Terminator Regions Regulates the Transcription Enhancement Potential of an Intron.Frontiers in molecular biosciences · 2021Article
- Transcriptional regulation of genes bearing intronic heterochromatin in the rice genome.PLoS genetics · 2020Article
- The novel geneHorticulture research · 2020Article
- Lose-of-Function of a Rice Nucleolus-Localized Pentatricopeptide Repeat Protein Is Responsible for the floury endosperm14 Mutant Phenotypes.Rice (New York, N.Y.) · 2019Article
- Selection of Reference Genes for qPCR Analyses of Gene Expression in Ramie Leaves and Roots across Eleven Abiotic/Biotic Treatments.Scientific reports · 2019Article
- Genome-Wide Transcription Factor Binding in Leaves from CThe Plant cell · 2019Article
- Introns as Gene Regulators: A Brick on the Accelerator.Frontiers in genetics · 2018Article
- Intron DNA Sequences Can Be More Important Than the Proximal Promoter in Determining the Site of Transcript Initiation.The Plant cell · 2017Article
- Intron sequences that stimulate gene expression in Arabidopsis.Plant molecular biology · 2016Article
- Characterization and putative post-translational regulation of α- and β-tubulin gene families in Salix arbutifolia.Scientific reports · 2016Article
- Article
- Intron-Mediated Enhancement: A Tool for Heterologous Gene Expression in Plants?Frontiers in plant science · 2016Review
- P1 Epigenetic Regulation in Leaves of High Altitude Maize Landraces: Effect of UV-B Radiation.Frontiers in plant science · 2016Article
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6 authors.
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Abstract
The genomic clone encoding an alpha-tubulin, OsTubA1, has been isolated from rice (Oryza sativa L.). The gene consists of four exons and three introns. RNA-blot analysis showed that the gene is strongly expressed in actively dividing tissues, including root tips, young leaves, and young flowers. Analysis of chimeric fusions between OsTubA1 and beta-glucuronidase (GUS) revealed that the intron 1 was required for high-level GUS expression in actively dividing tissues, corresponding with normal expression pattern of OsTubA1. Fusion constructs lacking the intron 1 showed more GUS staining in mature tissues rather than young tissues. When the intron 1 was placed at the distal region from 5'-upstream region or at the 3'-untranslated region, no enhancement of GUS expression was observed. Sequential deletions of the OsTubA1 intron 1 brought about a gradual reduction of GUS activity in calli. These results suggest that tissue-preferential expression of the OsTubA1 gene is mediated by the intron 1 and that it may be involved in a mechanism for an efficient RNA splicing that is position dependent.
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