ArticleMolecular biology of the cell2000
Luv1p/Rki1p/Tcs3p/Vps54p, a yeast protein that localizes to the late Golgi and early endosome, is required for normal vacuolar morphology.
Article in Molecular biology of the cell, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 53 citations in OpenAlex.
- GARP Complex in Golgi Physiology.Sub-cellular biochemistry · 2026Review
- Role of GARP Vesicle Tethering Complex in Golgi Physiology.International journal of molecular sciences · 2023Review
- Vps54 Regulates Lifespan and Locomotor Behavior in AdultFrontiers in genetics · 2021Article
- Vps54 regulatesBiology open · 2020Article
- Hygromycin B hypersensitive (hhy) mutants implicate an intact trans-Golgi and late endosome interface in efficient Tor1 vacuolar localization and TORC1 function.Current genetics · 2017Article
- Cardiolipin Regulates Mitophagy through the Protein Kinase C Pathway.The Journal of biological chemistry · 2017Article
- Article
- VPS54 and the wobbler mouse.Frontiers in neuroscience · 2015Review
- The protein transportation pathway from Golgi to vacuoles via endosomes plays a role in enhancement of methylmercury toxicity.Scientific reports · 2014Article
- The Caenorhabditis elegans GARP complex contains the conserved Vps51 subunit and is required to maintain lysosomal morphology.Molecular biology of the cell · 2011Article
- Identification of yeast genes involved in k homeostasis: loss of membrane traffic genes affects k uptake.G3 (Bethesda, Md.) · 2011Article
- New regulators of a high affinity Ca2+ influx system revealed through a genome-wide screen in yeast.The Journal of biological chemistry · 2011Article
- Transport according to GARP: receiving retrograde cargo at the trans-Golgi network.Trends in cell biology · 2011Review
- Association of calcineurin with the COPI protein Sec28 and the COPII protein Sec13 revealed by quantitative proteomics.PloS one · 2011Article
- Vesicle docking to the spindle pole body is necessary to recruit the exocyst during membrane formation in Saccharomyces cerevisiae.Molecular biology of the cell · 2010Article
- Genomic analysis of severe hypersensitivity to hygromycin B reveals linkage to vacuolar defects and new vacuolar gene functions in Saccharomyces cerevisiae.Current genetics · 2010Article
- Involvement of vacuolar sequestration and active transport in tolerance of Saccharomyces cerevisiae to hop iso-alpha-acids.Applied and environmental microbiology · 2010Article
- Dual roles of the mammalian GARP complex in tethering and SNARE complex assembly at the trans-golgi network.Molecular and cellular biology · 2009Article
- A novel immunodetection screen for vacuolar defects identifies a unique allele of VPS35 in S. cerevisiae.Molecular and cellular biochemistry · 2008Article
- Yarrowia lipolytica vesicle-mediated protein transport pathways.BMC evolutionary biology · 2007Article
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2 authors at 1 institution in 1 country.
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Abstract
We have characterized LUV1/RKI1/TCS3/VPS54, a novel yeast gene required to maintain normal vacuolar morphology. The luv1 mutant was identified in a genetic screen for mutants requiring the phosphatase calcineurin for vegetative growth. luv1 mutants lack a morphologically intact vacuole and instead accumulate small vesicles that are acidified and contain the vacuolar proteins alkaline phosphatase and carboxypeptidase Y and the vacuolar membrane H(+)-ATPase. Endocytosis appears qualitatively normal in luv1 mutants, but some portion (28%) of carboxypeptidase Y is secreted. luv1 mutants are sensitive to several ions (Zn(2+), Mn(2+), and Cd(2+)) and to pH extremes. These mutants are also sensitive to hygromycin B, caffeine, and FK506, a specific inhibitor of calcineurin. Some vacuolar protein-sorting mutants display similar drug and ion sensitivities, including sensitivity to FK506. Luv1p sediments at 100,000 x g and can be solubilized by salt or carbonate, indicating that it is a peripheral membrane protein. A Green Fluorescent Protein-Luv1 fusion protein colocalizes with the dye FM 4-64 at the endosome, and hemagglutinin-tagged Luv1p colocalizes with the trans-Golgi network/endosomal protease Kex2p. Computer analysis predicts a short coiled-coil domain in Luv1p. We propose that this protein maintains traffic through or the integrity of the early endosome and that this function is required for proper vacuolar morphology.
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