ArticleMolecular biology of the cell2000
Transient nucleolar localization Of U6 small nuclear RNA in Xenopus Laevis oocytes.
Article in Molecular biology of the cell, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 71 citations in OpenAlex.
- A guide to the biogenesis and functions of endogenous small non-coding RNAs in animals.Nature reviews. Molecular cell biology · 2025Review
- Nup107 contributes to the maternal-to-zygotic transition by preventing the premature nuclear export of pri-miR427.Development (Cambridge, England) · 2025Article
- Phosphorylation of a nuclear condensate regulates cohesion and mRNA retention.Nature communications · 2025Article
- The life of U6 small nuclear RNA, from cradle to grave.RNA (New York, N.Y.) · 2018Review
- Plant snRNP Biogenesis: A Perspective from the Nucleolus and Cajal Bodies.Frontiers in plant science · 2017Review
- Proteomic characterization of the nucleolar linker histone H1 interaction network.Journal of molecular biology · 2015Article
- A role for the Cajal-body-associated SUMO isopeptidase USPL1 in snRNA transcription mediated by RNA polymerase II.Journal of cell science · 2014Article
- Article
- Periodic expression of Sm proteins parallels formation of nuclear Cajal bodies and cytoplasmic snRNP-rich bodies.Histochemistry and cell biology · 2011Article
- Arginine methylation analysis of the splicing-associated SR protein SFRS9/SRP30C.Cellular & molecular biology letters · 2009Article
- TRF4 is involved in polyadenylation of snRNAs in Drosophila melanogaster.Molecular and cellular biology · 2008Article
- The assembly of a spliceosomal small nuclear ribonucleoprotein particle.Nucleic acids research · 2008Review
- 3'-cyclic phosphorylation of U6 snRNA leads to recruitment of recycling factor p110 through LSm proteins.RNA (New York, N.Y.) · 2008Article
- Different mechanisms for pseudouridine formation in yeast 5S and 5.8S rRNAs.Molecular and cellular biology · 2008Article
- The role of the plant nucleolus in pre-mRNA processing.Current topics in microbiology and immunology · 2008Review
- Subcellular localization of CIAPIN1.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2006Article
- Review
- Identification, cloning, and functional analysis of the human U6 snRNA-specific terminal uridylyl transferase.RNA (New York, N.Y.) · 2006Article
- Spatial organization of transcription by RNA polymerase III.Nucleic acids research · 2006Review
- U4 snRNA nucleolar localization requires the NHPX/15.5-kD protein binding site but not Sm protein or U6 snRNA association.The Journal of cell biology · 2003Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent studies on the 2'-O-methylation and pseudouridylation of U6 small nuclear RNA (snRNA) hypothesize that these posttranscriptional modifications might occur in the nucleolus. In this report, we present direct evidence for the nucleolar localization of U6 snRNA and analyze the kinetics of U6 nucleolar localization after injection of in vitro transcribed fluorescein-labeled transcripts into Xenopus laevis oocytes. In contrast to U3 small nucleolar RNA (snoRNA) which developed strong nucleolar labeling over 4 h and maintained strong nucleolar signals through 24 h, U6 snRNA localized to nucleoli immediately after injection, but nucleolar staining decreased after 4 h. By 24 h after injection of U6 snRNA, only weak nucleolar signals were observed. Unlike the time-dependent profile of strong nucleolar localization of U6 snRNA or U3 snoRNA, injection of fluorescein-labeled U2 snRNA gave weak nucleolar staining at all times throughout a 24-h period; U2 snRNA modifications are believed to occur outside of the nucleolus. The notion that the decrease of U6 signals over time was due to its trafficking out of nucleoli and not to transcript degradation was supported by the demonstration of U6 snRNA stability over time. Therefore, in contrast to snoRNAs like U3, U6 snRNA transiently passes through nucleoli.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.