ArticleMolecular biology of the cell2000
Reversible merger of membranes at the early stage of influenza hemagglutinin-mediated fusion.
Article in Molecular biology of the cell, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
- Cyclosporine A rescues the influenza virus fusion with IFITM3-expressing cells by relocating the restriction factor to intraluminal vesicles of multivesicular bodies.Journal of virology · 2026Article
- Viral glycoprotein-mediated entry and antibody-mediated immunity in HIV-1 and SARS-CoV-2 infection.Frontiers in immunology · 2025Review
- Cell fusion dynamics: mechanisms of multinucleation in osteoclasts and macrophages.Inflammation and regeneration · 2024Review
- Myomerger promotes fusion pore by elastic coupling between proximal membrane leaflets and hemifusion diaphragm.Nature communications · 2021Article
- The regulatory role of Myomaker and Myomixer-Myomerger-Minion in muscle development and regeneration.Cellular and molecular life sciences : CMLS · 2020Review
- Myomaker and Myomerger Work Independently to Control Distinct Steps of Membrane Remodeling during Myoblast Fusion.Developmental cell · 2018Article
- Calculating Transition Energy Barriers and Characterizing Activation States for Steps of Fusion.Biophysical journal · 2016Article
- Reovirus FAST Proteins Drive Pore Formation and Syncytiogenesis Using a Novel Helix-Loop-Helix Fusion-Inducing Lipid Packing Sensor.PLoS pathogens · 2015Article
- Lysophosphatidylcholine reversibly arrests pore expansion during syncytium formation mediated by diverse viral fusogens.Journal of virology · 2014Article
- The transmembrane domain and acidic lipid flip-flop regulates voltage-dependent fusion mediated by class II and III viral proteins.PloS one · 2013Article
- IFITM proteins restrict viral membrane hemifusion.PLoS pathogens · 2013Article
- Negative potentials across biological membranes promote fusion by class II and class III viral proteins.Molecular biology of the cell · 2010Article
- Prm1 functions as a disulfide-linked complex in yeast mating.The Journal of biological chemistry · 2010Article
- Common principles and intermediates of viral protein-mediated fusion: the HIV-1 paradigm.Retrovirology · 2008Review
- Mechanics of membrane fusion.Nature structural & molecular biology · 2008Review
- Synthesis and characterization of stable fluorocarbon nanostructures as drug delivery vehicles for cytolytic peptides.Nano letters · 2008Article
- Fusion induced by a class II viral fusion protein, semliki forest virus E1, is dependent on the voltage of the target cell.Journal of virology · 2007Article
- Membrane hemifusion is a stable intermediate of exocytosis.Developmental cell · 2007Article
- Heterogeneity of early intermediates in cell-liposome fusion mediated by influenza hemagglutinin.Biophysical journal · 2006Article
- Membranes of the world unite!The Journal of cell biology · 2006Review
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fusion mediated by influenza hemagglutinin (HA), a prototype fusion protein, is commonly detected as lipid and content mixing between fusing cells. Decreasing the surface density of fusion-competent HA inhibited these advanced fusion phenotypes and allowed us to identify an early stage of fusion at physiological temperature. Although lipid flow between membranes was restricted, the contacting membrane monolayers were apparently transiently connected, as detected by the transformation of this fusion intermediate into complete fusion after treatments known to destabilize hemifusion diaphragms. These reversible connections disappeared within 10-20 min after application of low pH, indicating that after the energy released by HA refolding dissipated, the final low pH conformation of HA did not support membrane merger. Although the dynamic character and the lack of lipid mixing at 37 degrees C distinguish the newly identified fusion intermediate from the intermediate arrested at 4 degrees C described previously, both intermediates apparently belong to the same family of restricted hemifusion (RH) structures. Because the formation of transient RH structures at physiological temperatures was as fast as fusion pore opening and required less HA, we hypothesize that fusion starts with the formation of multiple RH sites, only a few of which then evolve to become expanding fusion pores.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.