ArticleMolecular biology of the cell2000
The APC11 RING-H2 finger mediates E2-dependent ubiquitination.
Article in Molecular biology of the cell, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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Who cites it
61 citing papers in PubMed, 171 citations in OpenAlex.
- The Anaphase-Promoting Complex/Cyclosome Is a Cellular Ageing Regulator.International journal of molecular sciences · 2022Review
- SAMBA controls cell division rate during maize development.Plant physiology · 2022Article
- CDC20 promotes bone formation via APC/C dependent ubiquitination and degradation of p65.EMBO reports · 2021Article
- In silico analysis and molecular identification of an anaphase-promoting complex homologue from human pathogen Entamoeba histolytica.Journal, genetic engineering & biotechnology · 2021Article
- A unique binding mode of Nek2A to the APC/C allows its ubiquitination during prometaphase.EMBO reports · 2020Article
- Posing the APC/C E3 Ubiquitin Ligase to Orchestrate Cell Division.Trends in cell biology · 2019Review
- APC/C: current understanding and future perspectives.F1000Research · 2019Review
- Visualizing the complex functions and mechanisms of the anaphase promoting complex/cyclosome (APC/C).Open biology · 2017Review
- Atomic-Resolution Structures of the APC/C Subunits Apc4 and the Apc5 N-Terminal Domain.Journal of molecular biology · 2015Article
- Cyclin B3 controls anaphase onset independent of spindle assembly checkpoint in meiotic oocytes.Cell cycle (Georgetown, Tex.) · 2015Article
- Trichomes as models for studying plant cell differentiation.Cellular and molecular life sciences : CMLS · 2013Review
- Expression Profile of Penaeus monodon Ubiquitin Conjugating Enzyme (PmUbc) at Protein Level in White spot syndrome virus Challenged Shrimp.Indian journal of virology : an official organ of Indian Virological Society · 2013Article
- Tripartite motif ligases catalyze polyubiquitin chain formation through a cooperative allosteric mechanism.The Journal of biological chemistry · 2013Article
- Dma/RNF8 proteins are evolutionarily conserved E3 ubiquitin ligases that target septins.Cell cycle (Georgetown, Tex.) · 2013Article
- APC15 mediates CDC20 autoubiquitylation by APC/C(MCC) and disassembly of the mitotic checkpoint complex.Nature structural & molecular biology · 2012Article
- Dnt1 acts as a mitotic inhibitor of the spindle checkpoint protein dma1 in fission yeast.Molecular biology of the cell · 2012Article
- Dephosphorylation of Cdc20 is required for its C-box-dependent activation of the APC/C.The EMBO journal · 2012Article
- lemmingA encodes the Apc11 subunit of the APC/C in Drosophila melanogaster that forms a ternary complex with the E2-C type ubiquitin conjugating enzyme, Vihar and Morula/Apc2.Cell division · 2012Article
- Structural insights into anaphase-promoting complex function and mechanism.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2011Review
- APC15 drives the turnover of MCC-CDC20 to make the spindle assembly checkpoint responsive to kinetochore attachment.Nature cell biology · 2011Article
1 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Polyubiquitination marks proteins for degradation by the 26S proteasome and is carried out by a cascade of enzymes that includes ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s). The anaphase-promoting complex or cyclosome (APC/C) comprises a multisubunit ubiquitin ligase that mediates mitotic progression. Here, we provide evidence that the Saccharomyces cerevisiae RING-H2 finger protein Apc11 defines the minimal ubiquitin ligase activity of the APC. We found that the integrity of the Apc11p RING-H2 finger was essential for budding yeast cell viability, Using purified, recombinant proteins we showed that Apc11p interacted directly with the Ubc4 ubiquitin conjugating enzyme (E2). Furthermore, purified Apc11p was capable of mediating E1- and E2-dependent ubiquitination of protein substrates, including Clb2p, in vitro. The ability of Apc11p to act as an E3 was dependent on the integrity of the RING-H2 finger, but did not require the presence of the cullin-like APC subunit Apc2p. We suggest that Apc11p is responsible for recruiting E2s to the APC and for mediating the subsequent transfer of ubiquitin to APC substrates in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.