ArticleMolecular biology of the cell2000
Accumulation of rab4GTP in the cytoplasm and association with the peptidyl-prolyl isomerase pin1 during mitosis.
Article in Molecular biology of the cell, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 41 citations in OpenAlex.
- Pin1-catalyzed conformational regulation after phosphorylation: A distinct checkpoint in cell signaling and drug discovery.Science signaling · 2024Review
- Small RAB GTPases Regulate Multiple Steps of Mitosis.Frontiers in cell and developmental biology · 2016Review
- Rab11, but not Rab4, facilitates cyclic AMP- and tauroursodeoxycholate-induced MRP2 translocation to the plasma membrane.American journal of physiology. Gastrointestinal and liver physiology · 2014Article
- The R-Ras/RIN2/Rab5 complex controls endothelial cell adhesion and morphogenesis via active integrin endocytosis and Rac signaling.Cell research · 2012Article
- Protein kinase Cδ differentially regulates cAMP-dependent translocation of NTCP and MRP2 to the plasma membrane.American journal of physiology. Gastrointestinal and liver physiology · 2012Article
- Molecular mechanisms of SERT in platelets: regulation of plasma serotonin levels.Molecular interventions · 2010Review
- Neuron specific Rab4 effector GRASP-1 coordinates membrane specialization and maturation of recycling endosomes.PLoS biology · 2010Article
- Post-phosphorylation prolyl isomerisation of gephyrin represents a mechanism to modulate glycine receptors function.The EMBO journal · 2007Article
- Expression of RAB4B, a protein governing endocytic recycling, is co-regulated with MHC class II genes.Nucleic acids research · 2007Article
- A role for the Rab6A' GTPase in the inactivation of the Mad2-spindle checkpoint.The EMBO journal · 2006Article
- Rabip4' is an effector of rab5 and rab4 and regulates transport through early endosomes.Molecular biology of the cell · 2004Article
- The prolyl isomerase Pin1 in breast development and cancer.Breast cancer research : BCR · 2003Review
- Loss of Pin1 function in the mouse causes phenotypes resembling cyclin D1-null phenotypes.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Endothelial nitric oxide synthase activity is linked to its presence at cell-cell contacts.The Biochemical journal · 2002Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Transport through the endocytic pathway is inhibited during mitosis. The mechanism responsible for this inhibition is not understood. Rab4 might be one of the proteins involved as it regulates transport through early endosomes, is phosphorylated by p34(cdc2) kinase, and is translocated from early endosomes to the cytoplasm during mitosis. We investigated the perturbation of the rab4 GTPase cycle during mitosis. Newly synthesized rab4 was less efficiently targeted to membranes during mitosis. By subcellular fractionation of mitotic cells, we found a large increase of cytosolic rab4 in the active GTP-form, an increase not associated with the cytosolic rabGDP chaperone GDI. Instead, phosphorylated rab4 is in a complex with the peptidyl-prolyl isomerase Pin1 during mitosis, but not during interphase. Our results show that less efficient recruitment of rab4 to membranes and a bypass of the normal GDI-mediated retrieval of rab4GDP from early endosomes reduce the amount of rab4GTP on membranes during mitosis. We propose that phosphorylation of rab4 inhibits both the recruitment of rab4 effector proteins to early endosomes and the docking of rab4-containing transport vesicles. This mechanism might contribute to the inhibition of endocytic membrane transport during mitosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.