ArticleMolecular pathology : MP2000
Amplification of PCR products in excess of 600 base pairs using DNA extracted from decalcified, paraffin wax embedded bone marrow trephine biopsies.
Article in Molecular pathology : MP, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed.
- Spatial proteomics and transcriptomics characterization of tissue and multiple cancer types including decalcified marrow.Cancer biomarkers : section A of Disease markers · 2025Article
- High multiplex analysis of the immune microenvironment in bone marrow trephine samples using GeoMX™ digital spatial profiling.Immuno-oncology technology · 2020Article
- Mutational Screening of BRCA1/2 Genes as a Predictive Factor for Therapeutic Response in Epithelial Ovarian Cancer: A Consensus Guide from the Spanish Society of Pathology (SEAP-IAP) and the Spanish Society of Human Genetics (AEGH).Virchows Archiv : an international journal of pathology · 2020Review
- The Effectiveness of Mini Primer STR CODIS in DNA Degradation as the Effect of High-Temperature Exposure.Analytical cellular pathology (Amsterdam) · 2020Article
- Guidance for laboratories performing molecular pathology for cancer patients.Journal of clinical pathology · 2014Review
- Genotyping of DNA samples isolated from formalin-fixed paraffin-embedded tissues using preamplification.The Journal of molecular diagnostics : JMD · 2010Article
- Evaluation of 13q14 status in multiple myeloma by digital single nucleotide polymorphism technology.The Journal of molecular diagnostics : JMD · 2009Article
- The isolation of nucleic acids from fixed, paraffin-embedded tissues-which methods are useful when?PloS one · 2007Article
- Development and clinical application of a panfungal PCR assay to detect and identify fungal DNA in tissue specimens.Journal of clinical microbiology · 2007Article
- Mitochondrial DNA mutations in differentiated thyroid cancer with respect to the age factor.World journal of surgery · 2007Article
- Ancillary techniques in bone marrow pathology: molecular diagnostics on bone marrow trephine biopsies.Virchows Archiv : an international journal of pathology · 2005Review
- Application of BIOMED-2 primers in fixed and decalcified bone marrow biopsies: analysis of immunoglobulin H receptor rearrangements in B-cell non-Hodgkin's lymphomas.The Journal of molecular diagnostics : JMD · 2005Article
- Novel multipurpose methodology for detection of mycobacteria in pulmonary and extrapulmonary specimens by smear microscopy, culture, and PCR.Journal of clinical microbiology · 2005Article
- Review
- Formic acid decalcification of bone marrow trephines degrades DNA: alternative use of EDTA allows the amplification and sequencing of relatively long PCR products.Molecular pathology : MP · 2000Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimsTo establish a robust method of extracting DNA from paraffin wax embedded bone marrow trephine (PBMT) biopsies for the amplification of relatively long polymerase chain reaction (PCR) products.
methodXylene and ethanol were used to remove paraffin wax from eight formalin fixed, EDTA decalcified PBMT biopsies and DNA extraction was performed using a Qiagen QIAamp tissue kit. The DNA samples were amplified using nine different PCR primers sets, including those used to detect chromosomal translocations (t(11;14) and t(14;18), and clonal B cell populations. A t(11;14) PCR product of approximately 600 base pairs (bp) was sequenced using dye terminator cycle sequencing.
resultsAll eight DNA samples extracted from PBMT biopsies were amplified successfully to generate DNA fragments up to 643 bp in length. Chromosomal translocations and immunoglobulin gene rearrangements were detected by PCR in some of the samples. Sequencing of the t(11;14) PCR product demonstrated the presence of chimaeric sequences, which included both bcl-1 and immunoglobulin heavy chain (IgH) gene sequences, consistent with the presence of this translocation.
conclusionsThis method enables PCR analyses of PBMT biopsies that were not previously possible, offering the prospect of improved accuracy of diagnosis and the monitoring of patients with bone marrow disease.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.