ArticleProceedings of the National Academy of Sciences of the United States of America2000
V(D)J recombination is not activated by demethylation of the kappa locus.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 39 citations in OpenAlex.
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- Trim24 and Trim33 Play a Role in Epigenetic Silencing of Retroviruses in Embryonic Stem Cells.Viruses · 2020Article
- Article
- Epigenetic Control of B Cell Development and B-Cell-Related Immune Disorders.Clinical reviews in allergy & immunology · 2016Review
- The Interplay between Chromatin and Transcription Factor Networks during B Cell Development: Who Pulls the Trigger First?Frontiers in immunology · 2014Review
- Natural resistance-associated macrophage protein is a cellular receptor for sindbis virus in both insect and mammalian hosts.Cell host & microbe · 2011Article
- Hematopoiesis and immunity of HOXB4-transduced embryonic stem cell-derived hematopoietic progenitor cells.Blood · 2008Article
- Cis-regulatory elements and epigenetic changes control genomic rearrangements of the IgH locus.Advances in immunology · 2008Review
- Critical roles of the immunoglobulin intronic enhancers in maintaining the sequential rearrangement of IgH and Igk loci.The Journal of experimental medicine · 2006Article
- Role of B-cell proliferation in the establishment of gammaherpesvirus latency.Journal of virology · 2005Article
- Effect of CpG methylation on RAG1/RAG2 reactivity: implications of direct and indirect mechanisms for controlling V(D)J cleavage.EMBO reports · 2003Article
- Activation of the early B-cell-specific mb-1 (Ig-alpha) gene by Pax-5 is dependent on an unmethylated Ets binding site.Molecular and cellular biology · 2003Article
- Modifications of histone cores and tails in V(D)J recombination.Genome biology · 2003Review
- Novel retroviral vectors to facilitate expression screens in mammalian cells.Nucleic acids research · 2002Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
V(D)J recombination is thought to be regulated by changes in the accessibility of target sites, such as modulation of methylation. To test whether demethylation of the kappa locus can activate recombination, we generated two recombinationally active B cell lines in which the gene for maintenance of genomic DNA methylation, Dnmt1, was flanked with loxP sites. Transduction with a retrovirus expressing both the cre recombinase and green fluorescent protein allowed us to purify recombinationally active cells devoid of methylation. Loss of methylation of the kappa locus was not sufficient to activate recombination, although transcription was activated in one line. It appears that demethylation of the kappa locus is not the rate-limiting step for altering accessibility and thus regulated demethylation does not generate specificity of recombination.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.